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Murashige and Skoog Plant Cell Culture Media Formulations

Much of plant tissue culture research today still utilizes media developed many years ago by Murashige and Skoog. Below you will find formulation information for many of these media.

Basal Salts Mixtures

M5524 – Murashige and Skoog basal salt mixture (MS)
M0654 – Murashige and Skoog basal salt macronutrient solution
M0529 – Murashige and Skoog basal salt micronutrient solution
M2909 – Murashige and Skoog modified basal salt mixture

Component (mg/L)M5524M0654M0529M2909
Aluminum chloride • 6H2O
Ammonium nitrate1650.01650.0
Ammonium phosphate monobasic
Ammonium sulfate
Boric acid6.2 6.26.2
Calcium chloride anhydrous332.2332.2 332.2
Calcium nitrate
Calcium phosphate tribasic
Cobalt chloride • 6H2O0.025 0.0250.025
Cupric sulfate • 5H2O0.025 0.0250.025
Na2-EDTA37.26 37.337.26
ZnNa2-EDTA
Ferric chloride • 6H2O
Ferric citrate
Ferric sulfate
Ferric tartrate • 2H2O
Ferrous sulfate • 7H2O27.8 27.827.8
Magnesium sulfate180.7180.7 180.7
Manganese chloride • 4H2O
Manganese sulfate • H2O16.9 16.916.9
Molybdenum trioxide
Molybdic acid (sodium salt) • 2H2O0.25 0.250.25
Nickel chloride • 6H2O
Nickel sulfate • 6H2O
Potassium chloride
Potassium iodide0.83 0.830.83
Potassium nitrate1900.01900.0 1900.0
Potassium phosphate monobasic170.0170.0 170.0
Potassium sulfate
Sodium nitrate
Sodium phosphate monobasic
Sodium sulfate
Zinc nitrate • 6H2O
Zinc sulfate • 7H2O8.6 8.68.6
Organics (mg/L)M5524M0654M0529M2909
Activated charcoal
Adenine hemisulfate
Agar
6-Benzylaminopurine (BA)
D-Biotin
6gamma-gamma-
Dimethylallylaminopurine
Glycine (free base)
Indole-3-acetic acid
Indole-3-butyric acid
Kinetin
MES (free acid)
myo-Inositol
alpha-Naphthaleneacetic acid
Nictotinic acid (free acid)
Peptone
Pyridoxine • HCl
Sucrose
Thiamine • HCl
M5524M0654M0529M2909
Grams of powder to prepare 1 L4.3n/an/a2.7
pH ± 0.5 at room temperature3.94.33.23.9
Component (mg/L)M6899M5519M0404M9274M7024
Aluminum chloride • 6H2O
Ammonium nitrate1650.01650.01650.01650.01650.0
Ammonium phosphate monobasic
Ammonium sulfate
Boric acid6.26.26.26.26.2
Calcium chloride anhydrous332.2332.2332.2332.2332.2
Calcium nitrate
Calcium phosphate tribasic
Cobalt chloride • 6H2O0.0250.0250.0250.0250.025
Cupric sulfate • 5H2O0.0250.0250.0250.0250.025
Na2-EDTA37.2637.2637.2637.2637.26
ZnNa2-EDTA
Ferric chloride • 6H2O
Ferric citrate
Ferric sulfate
Ferric tartrate • 2H2O
Ferrous sulfate • 7H2O27.827.827.827.827.8
Magnesium sulfate180.7180.7180.7180.7180.7
Manganese chloride • 4H2O
Manganese sulfate • H2O16.916.916.916.916.9
Molybdenum trioxide
Molybdic acid (sodium salt) • 2H2O0.250.250.250.250.25
Nickel chloride • 6H2O
Nickel sulfate • 6H2O
Potassium chloride
Potassium iodide0.830.830.830.830.83
Potassium nitrate1900.01900.01900.01900.01900.0
Potassium phosphate monobasic170.0170.0170.0170.0170.0
Potassium sulfate
Sodium nitrate
Sodium phosphate monobasic 148.0
Sodium sulfate
Zinc nitrate • 6H2O
Zinc sulfate • 7H2O8.68.68.68.68.6
Organics (mg/L)M6899M5519M0404M9274M7024
Activated charcoal
Adenine hemisulfate 80.0
Agar 8000.0
6-Benzylaminopurine (BA)
D-Biotin
6gamma-gamma-
Dimethylallylaminopurine
30.0
Glycine (free base) 2.0 2.0
Indole-3-acetic acid 0.3
Indole-3-butyric acid
Kinetin
MES (free acid)
myo-Inositol100.0100.0100.0100.0100.0
alpha-Naphthaleneacetic acid
Nictotinic acid (free acid) 0.51.00.5
Peptone
Pyridoxine • HCl 0.51.00.5
Sucrose 30,00030,000
Thiamine • HCl0.40.110.00.10.4
M6899M5519M0404M9274M7024
Grams of powder to prepare 1 L4.44.44.442.434.7
pH ± 0.5 at room temperature3.93.93.85.03.9
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