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HomeSolid Phase Microextraction (SPME)Standard Methods 6040D: GC Analysis of Geosmin and 2-MIB on SLB®-5ms after SPME using 50/30 µm DVB/Carboxen/PDMS Fiber

Standard Methods 6040D: GC Analysis of Geosmin and 2-MIB on SLB®-5ms after SPME using 50/30 µm DVB/Carboxen/PDMS Fiber

Standard Methods 6040D: GC Analysis of Geosmin and 2-MIB on SLB®-5ms after SPME using 50/30 μm DVB/Carboxen/PDMS Fiber

Conditions

desorption process260 °C for 3 min.
extractionheadspace, 65 °C (30 min)
SPME fiber2 cm Metal coated with 50/30 µm DVB/Carboxen/PDMS (57914-U)
columnSLB-5ms, 30 m x 0.25 mm I.D., 0.25 µm (28471-U)
oven60 °C (2 min), 8 °C/min to 200 °C
carrier gashelium, 1 mL/min constant
sample20/10 ppt odor compounds in 25 mL water + 25% NaCl
liner0.75 mm I.D., SPME
MSD interface300 °C
scan rangeSIM, m/z = 137, 124, 95, 112

Description

CategoriesAnalytical Chromatography, SPME Applications, GC Applications, Volatiles
Featured IndustryEnvironmental
Food and Beverages
Legal InformationSLB is a registered trademark of Sigma-Aldrich Co. LLC
Other NotesComplementary products:
Syringes
Vials
Gas Purification and Gas Management
Inlet Liners, Glass Wool, and Specialized Hand Tools
Septa and Specialized Hand Tools
Column Ferrules, Nuts, and Specialized Hand Tools
suitabilityapplication for SPME, application for GC

Analyte

  • (±)-Geosmin
  • 2-Isobutyl-3-methoxypyrazine
  • 2-Isopropyl-3-methoxypyrazine
  • 2-Methylisoborneol
Materials
Product No.Product NameDescriptionPricing
SCR005Disolución Accutase para separación de célulasA cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.
MAB5326Anticuerpo anti-nestina, clon 10C2clone 10C2, Chemicon®, from mouse
MAB4401Anti-Oct-4 Antibody, clone 10H11.2clone 10H11.2, Chemicon®, from mouse
MAB4423Anti-SOX-2 Antibody, clone 10H9.1clone 10H9.1, from mouse
MAB4360Anticuerpo anti-TRA-1-60, clon TRA-1-60clone TRA-1-60, Chemicon®, from mouse
SCM004ENStem-A Neural Expansion MediumThe ENStem-A Neural Expansion Medium is a defined serum-free formulation that has been optimized for the culture & expansion of ENStem-A Human Neural Progenitor Cells.
SCC058FibroGRO® Xeno-Free Human Foreskin FibroblastsThe FibroGRO Xeno-Free Human Foreskin Fibroblasts are derived from normal human foreskin & have been isolated & manufactured under xeno-free conditions.
GF446HumanKine® Thermostable bFGF,
Human Recombinant
Engineered bFGF growth factor with enchanced thermostability compared to the wild-type bFGF protein. Allows a less vigorous feeding schedule for human es/ips and neural stem cell culture.
SCR544Human STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming KitThe Human STEMCCA Constitutive Polycistronic Lentivirus Kit contains high titer polycistronic lentivirus & Polybrene transfection reagent that have been validated for the generation of human iPS cells from human foreskin fibroblasts.
SCM133Solución PluriSTEM® Dispasa-IIDispase-II has proven to be a rapid and effective, yet gentle, agent for separating many tissues and cells grown in vitro.
SCM130PluriSTEM® Human ES/iPS Cell MediumDefined small molecule based serum-free medium that enables feeder-free culture of human ES/iPS cells and allows for media exchanges every other day without compromising the morphology or long term functionality of human pluripotent stem cells.
SCM132PluriSTEM-XF® Human ES/iPS Cell MediumPluriSTEM-XF Human ES/iPS Medium is a complete xeno-free medium formulation for the feeder-free culture of human ES and iPS cells.
CC130PluriSTEM-XF® Recombinant VitronectinHuman pluripotent stem cell culture.
SCR550Simplicon® RNA Reprogramming Kit (OKSG)
SCR508TAT-recombinasa CreTAT-CRE Recombinase is a recombinant cell-permeant fusion cre-recombinase protein consisting of TAT sequence, a nuclear localization sequence (NLS) and it is known to catalyze the site specific recombination event between two loxP DNA sites.
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