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HPLC Analysis of Bisphenol A, Dimethylbisphenol A and Nonylphenol on Ascentis® Express RP-Amide (UV Detection)

HPLC Analysis of Bisphenol A, Dimethylbisphenol A and Nonylphenol on Ascentis® Express RP-Amide (UV Detection) application for HPLC

Materials

analytical column

Product No.
Description
Pricing

53922-U

Ascentis® Express RP-Amide, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 4.6 mm

standard

Product No.
Description
Pricing

33629

Alkylphenol Kit for DIN EN ISO 18857-2

analytical standard

CONDITIONS

column

Ascentis Express RP-Amide, 5 cm x 4.6 mm I.D., 2.7 μm particles (53922-U)

mobile phase

[A] water; [B] acetonitrile

gradient

50 to 100% B in 10 min

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 280 nm

injection

10 μL

sample

50 mg/L of each in 50:50, water: acetonitrile

Description

Analysis Note

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of milk thistle related compounds

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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