HPLC Analysis MAb Variants on Proteomix^® SCX-NP5, Fast Analysis

column

Proteomix SCX-NP5, 25 cm x 4.6 mm I.D., 5 μm particles (Z777164)

mobile phase

[A] 2.4 mM Tris, 1.5 mM imidazole, 11.6 mM piperazine, pH 6.0; [B] A + 0.5 M NaCl, pH 10.5

gradient

top (0.8 mL/min): 5% B held for 5 min; to 29.5% B in 35 min; bottom (1.5 mL/min): 5% B held for 2.7 min; to 29.5% B in 18.6 min

flow rate

0.8 mL/min or 1.5 mL/min

detector

UV 280 nm

injection

20 μL

sample

MAb 321, 5 mg/mL