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Key Documents

ES-E14TG2a

08021401, mouse embryo (blastocyst). Monolayer of spheroidal cells on feeder layer of PMEFs or STOs

Synonym(s):

E14TG2a Cell Line

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About This Item

UNSPSC Code:
41106514

product name

ES-E14TG2a, 08021401, from mouse embryo(blastocyst)

biological source

mouse embryo (blastocyst)

growth mode

Adherent

karyotype

Not specified

morphology

Adherent monolayer of spheroidal cells on feeder layer of mouse primary embryonic fibroblasts or STO cells. The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer.

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

Cell Line Origin

Mouse (strain 129/Ola) embryonic stem cell

Cell Line Description

Pluripotent mouse embryonic stem cell line. ES-E14TG2a cells are deficient in in HPRT and are resistant to 0.06mM 6-thioguanine.

DNA Profile

Not specified

Culture Medium

DMEM + 2 mM Glutamine + 1% NEAA + 0.1 mM 2-Mercaptoethanol + 1000 U/ml mouse inhibitory factor (LIF) + 1.5% sodium bicarbonate + 10% Fetal Bovine Serum. Renewal of medium every 2-3 days is required.

Subculture Routine

Not specified

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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