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Sigma-Aldrich

PIP5-Kinase HTRF Assay

The PIP5 Kinase HTRF Assay kit provides a method for assaying PI(4)P-5 kinases in a homogenous 384 well format. Suitable for screening of potential inhibitors of these enzymes.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.54

Quality Level

species reactivity

mouse, human

manufacturer/tradename

HTRF®
Upstate®

technique(s)

activity assay: suitable (kinase)

detection method

fluorometric (HTRF)

shipped in

dry ice

Application

Research Category
Apoptosis & Cancer
Research Sub Category
Oncology
The PIP5 Kinase HTRF Assay kit provides a method for assaying PI(4)P-5 kinases in a homogenous 384 well format. Suitable for screening of potential inhibitors of these enzymes.
Used to detect/quantify: PIP5 Kinase

Biochem/physiol Actions

Target Sub-Family: PI3K/PI4K

Packaging

Kit capacity: 384 wells

Components

Reaction Buffer (4X)

DM A Detection Reagent

DM C Detection Reagent

DM E Detection Reagent

Phosphatidylinositol 4-phosphate (1mM)

Phosphatidylserine (1mM)

Stop Reagent D

Storage and Stability

12 months at -70°C

Legal Information

HTRF is a registered trademark of Cis Bio International
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10-13 - German Storage Class 10 to 13


Certificates of Analysis (COA)

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Yuxun Zhang et al.
The Journal of biological chemistry, 292(24), 10239-10249 (2017-05-02)
SIRT5 is a lysine desuccinylase known to regulate mitochondrial fatty acid oxidation and the urea cycle. Here, SIRT5 was observed to bind to cardiolipin via an amphipathic helix on its N terminus. In vitro, succinyl-CoA was used to succinylate liver
Marco Spinazzi et al.
Nature protocols, 7(6), 1235-1246 (2012-06-02)
The assessment of mitochondrial respiratory chain (RC) enzymatic activities is essential for investigating mitochondrial function in several situations, including mitochondrial disorders, diabetes, cancer, aging and neurodegeneration, as well as for many toxicological assays. Muscle is the most commonly analyzed tissue

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