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Key Documents

R6767

SAFC

RPMI-1640 Medium

liquid, Modified, suitable for cell culture

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About This Item

MDL number:
UNSPSC Code:
12352207

sterility

sterile-filtered

form

liquid

IVD

for in vitro diagnostic use

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

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General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Application

Modified for Fragile X chromosome evaluation.

Formula variant

With sodium bicarbonate. Without folic acid and L-glutamine.

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