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M3678

SAFC

EX-CELL® MDCK Production Medium

Animal-component free, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Madin-Darby Canine Kidney Protein-free Medium

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About This Item

UNSPSC Code:
12352207

description

for research or for further manufacturing use

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

Application

Complete protein-free medium formulated to support the growth of Madin-Darby Canine Kidney (MDCK) cells for the growth and production of viruses.

Components

Contains inorganic salts, essential and non-essential amino acids, vitamins, recombinant human insulin and growth factors, other organic compounds, and trace elements.

Suitability

Designed and qualified for use in stringent BioManufacturing applications that requires the absence of both proteins and animal-derived components.

Reconstitution

Aseptically add 16.5 ml of 200 mM L-glutamine solution per liter of medium prior to use.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Yvonne Genzel et al.
Applied microbiology and biotechnology, 88(2), 461-475 (2010-07-10)
Over the last decade, adherent MDCK (Madin Darby canine kidney) and Vero cells have attracted considerable attention for production of cell culture-derived influenza vaccines. While numerous publications deal with the design and the optimization of corresponding upstream processes, one-to-one comparisons
Jonathan Liu et al.
Vaccine, 27(46), 6460-6463 (2009-06-30)
To develop a cell-based live attenuated influenza vaccine (LAIV) manufacturing process, several different cell lines were evaluated by comparing the titer of viruses after infection with LAIV strains. While several cell lines have been reported to support influenza virus replication
Manuel J Deutsch et al.
Journal of virology, 84(5), 2533-2546 (2009-12-25)
The Epstein-Barr virus efficiently infects human B cells. The EBV genome is maintained extrachromosomally and replicates synchronously with the host's chromosomes. The latent origin of replication (oriP) guarantees plasmid stability by mediating two basic functions: replication and segregation of the

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