E6635

Ethylenediaminetetraacetic acid disodium salt dihydrate

Sigma Grade, suitable for plant cell culture, 98.5-101.5%

• Synonym(s): Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na₂, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na₂
• Empirical Formula (Hill Notation): C₁₀H₁₄N₂Na₂O₈ · 2H₂O
• CAS Number: 6381-92-6
• Molecular Weight: 372.24
• Beilstein: 3900609
• EC Number: 205-358-3
• UNSPSC Code: 12352107
• PubChem Substance ID: 24894632
• NACRES: NA.77

Properties

• grade: Sigma Grade
• Quality Level: 200
• Assay: 98.5-101.5%
• form: powder
• reaction suitability: reagent type: chelator
• technique(s): cell culture | plant: suitable
• mp: 248 °C (dec.) (lit.)
• solubility: H₂O: soluble, clear, colorless
• SMILES string: O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
• InChI: 1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2
• InChI key: OVBJJZOQPCKUOR-UHFFFAOYSA-L

Description

General description

Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions, especially with cations to form an octahedral complex. Ethylenediaminetetraacetic acid disodium salt (EDTA) is a blood anticoagulant and contributes to the pathogenesis of pseudothrombocytopenia. It chelates with calcium in the blood and inhibits clotting and is routinely used in haematological tests. It enhances the antibacterial activity of lysozyme. EDTA used in chelation therapy chelates with calcium and favors dilation of artery, solubilisation of atheromatous plaques in atherosclerotic vascular disease. EDTA chelation therapy may also protect from oxidative damage during blood and lipid peroxidation in liver fibrosis.

Application

Ethylenediaminetetraacetic acid disodium salt dihydrate has been in seed germination trials of plant species and in protein extraction from Moss, Physcomitrella paten. It has also been used in lysis and vacuole buffer for the isolation of vacuoles from Petunia petals.

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Safety Information

• Pictograms: GHS07,GHS08
• Signal Word: Warning
• Hazard Statements: H332 - H373
• Precautionary Statements: P260 - P271 - P304 + P340 + P312 - P314 - P501
• Hazard Classifications: Acute Tox. 4 Inhalation - STOT RE 2 Inhalation
• Target Organs: Respiratory Tract
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves