Low Pressure Liquid Chromatography
Low-pressure liquid chromatography (LPLC) is an analytical technique that uses low pressure to drive a mobile phase through a column containing a stationary phase, separating complex mixtures by differential partitioning. Originally performed with open columns that used gravity to move the sample through the packing bed, it is also known as "open column liquid chromatography. The different LPLC modes allow for the precise and efficient purification of compounds by separating them based on their chemical properties, such as size, charge, or affinity.
It is mostly used for studying biomolecules like proteins, peptides, and monoclonal antibodies due to its non-destructive preparative nature. Typically, LPLC makes it possible to keep the sample for further studies. LPLC offers additional benefits such as a simple design, strong siphon capabilities, and modest instrumentation requirements including detectors, low-pressure pumps, and fraction collectors. Its versatility makes it indispensable across pharmaceuticals, biotechnology, food and beverage, environmental monitoring, and research sectors. Moreover, by using lower pressures and using less solvent, LPLC complies with the principles of green chemistry.
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Related Resources
- Brochure: Resins Selection Guide
This guide covers various chromatography methods including adsorption, affinity, gel filtration, hydrophobic interaction, and ion exchange chromatography.
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