Fast detection of alpha-fetoprotein-L3 using lens culinaris agglutinin immobilized gold nanoparticles.

Fast detection of alpha-fetoprotein-L3 using lens culinaris agglutinin immobilized gold nanoparticles.

Journal of nanoscience and nanotechnology (2014-04-18)

Ying Sun, Liubin Qin, Dejun Liu, Changhong Liu, Yongwei Sun, Yourong Duan

ABSTRACT

Colloidal gold is extensively used for molecular sensing because that the surface plasmon resonance (SPR) bands are affected by changes in the dielectric properties in the close vicinity of these structures due to the binding of ligands to the corresponding receptor molecules immobilized onto the nanostructures through chemi- or physisorption. We describe a simple method for the detection of Alpha-Fetoprotein-L3 which is a new generation of tumor marker for hepatocellular carcinoma (HCC) based on the aggregation of Lens culinaris agglutinin (LCA) Immobilized Gold Nanoparticles. The LCA conjugated GNPs obtained were 15-20 nm in diameter. The visible color change of the gold nanoparticles from purple to blue on interaction with 100 ng/mL of AFP-L3 is the principle applied here for the sensing of AFP-L3 level. UV/Vis spectroscopy also allows assay monitoring by quantifying the red shift of the plasmon resonance wavelength. With this method, the protein AFP-L3 can be rapidly detected as demanded for clinical diagnosis.

MATERIALS

Product Number

Brand

Product Description

265810

Sigma-Aldrich

Gold, foil, thickness 0.1 mm, 99.99% trace metals basis

265799

Sigma-Aldrich

Gold, wire, diam. 1.0 mm, 99.99% trace metals basis

265780

Sigma-Aldrich

Gold, wire, diam. 0.25 mm, 99.99% trace metals basis

326496

Sigma-Aldrich

Gold, foil, thickness 0.127 mm, 99.99% trace metals basis

349313

Sigma-Aldrich

Gold, wire, diam. 0.5 mm, 99.999% trace metals basis

268461

Sigma-Aldrich

Gold, foil, thickness 0.025 mm, 99.99% trace metals basis

255734

Sigma-Aldrich

Gold, powder, <850 μm, ≥99.99% trace metals basis

373184

Sigma-Aldrich

Gold, evaporation slug, diam. × L 0.3 cm × 0.6 cm, 99.99% trace metals basis

643254

Sigma-Aldrich

Gold coated glass cover slip, 99.999% (Au), layer thickness 100 Å, L × W × thickness 22 mm × 22 mm × 130-170 μm, square

643289

Sigma-Aldrich

Gold coated glass cover slip, 99.999% (Au), layer thickness 100 Å, diam. × thickness 15 mm × 130-170 μm

636347

Sigma-Aldrich

Gold, nanopowder, <100 nm particle size, 99.9% trace metals basis

643203

Sigma-Aldrich

Gold coated glass slide, 99.999% (Au), layer thickness 100 Å

643246

Sigma-Aldrich

Gold coated glass slide, 99.999% (Au), layer thickness 1000 Å

GF71627395

Gold, tube, 200mm, outside diameter 3.0mm, inside diameter 2.8mm, wall thickness 0.10mm, as drawn, 99.95%

GF54558175

Gold, insulated wire, 0.2m, conductor diameter 0.125mm, insulation thickness 0.016mm, PTFE (polytetrafluoroethylene) insulation, 99.99%

GF10186174

Gold, tube, 10mm, outside diameter 0.15mm, inside diameter 0.05mm, wall thickness 0.05mm, as drawn, 99.95%

GF37867887

Gold, rod, 50mm, diameter 1.6mm, as drawn, 99.95%

GF34898170

Gold, tube, 50mm, outside diameter 0.3mm, inside diameter 0.2mm, wall thickness 0.05mm, as drawn, 99.95%

GF55539524

Gold, tube, 100mm, outside diameter 1.0mm, inside diameter 0.7mm, wall thickness 0.15mm, as drawn, 99.95%

GF08843867

Gold, rod, 10mm, diameter 3.0mm, as drawn, 99.95%

GF58911206

Gold, tube, 200mm, outside diameter 0.3mm, inside diameter 0.2mm, wall thickness 0.05mm, as drawn, 99.95%

GF85112232

Gold, rod, 50mm, diameter 2.0mm, as drawn, 99.95%

GF48164531

Gold, tube, 100mm, outside diameter 2.0mm, inside diameter 1.7mm, wall thickness 0.15mm, as drawn, 99.95%

GF98454864

Gold, rod, 25mm, diameter 1.6mm, as drawn, 99.95%

GF86990350

Gold, tube, 100mm, outside diameter 2.0mm, inside diameter 1.8mm, wall thickness 0.1mm, as drawn, 99.95%

GF31080899

Gold, rod, 25mm, diameter 2.0mm, as drawn, 99.95%

GF77657574

Gold, sphere, 20pcs, diameter 0.5mm, 99.99%

GF47961243

Gold, tube, 50mm, outside diameter 2.0mm, inside diameter 1.7mm, wall thickness 0.15mm, as drawn, 99.95%

GF34099410

Gold, insulated wire, 2m, conductor diameter 0.125mm, insulation thickness 0.016mm, PTFE (polytetrafluoroethylene) insulation, 99.99%

GF20403638

Gold, insulated wire, 1m, conductor diameter 0.125mm, insulation thickness 0.014mm, polyester insulation, 99.99%