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  • The Design and Synthesis of Fluorescent Coumarin Derivatives and Their Study for Cu2+ Sensing with an Application for Aqueous Soil Extracts.

The Design and Synthesis of Fluorescent Coumarin Derivatives and Their Study for Cu2+ Sensing with an Application for Aqueous Soil Extracts.

Molecules (Basel, Switzerland) (2019-10-05)
Bin Qian, Linda Váradi, Adrian Trinchi, Suzie M Reichman, Lei Bao, Minbo Lan, Gang Wei, Ivan S Cole
ABSTRACT

A series of fluorescent coumarin derivatives 2a-e were systematically designed, synthesized and studied for their Cu2+ sensing performance in aqueous media. The sensitivities and selectivities of the on-to-off fluorescent Cu2+ sensing signal were in direct correlation with the relative arrangements of the heteroatoms within the coordinating moieties of these coumarins. Probes 2b and 2d exhibited Cu2+ concentration dependent and selective fluorescence quenching, with linear ranges of 0-80 μM and 0-10 μM, and limits of detection of 0.14 μM and 0.38 μM, respectively. Structural changes of 2b upon Cu2+ coordination were followed by fluorescence titration, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), mass spectrometry, and single crystal X-ray diffraction on the isolated Cu2+-coumarin complex. The results revealed a 1:1 stoichiometry between 2b and Cu2+, and that the essential structural features for Cu2+-selective coordination are the coumarin C=O and a three-bond distance between the amide NH and heterocyclic N. Probe 2b was also used to determine copper (II) levels in aqueous soil extracts, with recovery rates over 80% when compared to the standard soil analysis method: inductively coupled plasma-mass spectrometry (ICP-MS).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2-(Aminomethyl)benzimidazole dihydrochloride, 98%
Sigma-Aldrich
N-Boc-ethylenediamine, ≥98.0% (NT)
Sigma-Aldrich
2-Picolylamine, 99%
Sigma-Aldrich
Coumarin-3-carboxylic acid, 99%