MAB1476-I

Anti-Chymotrypsin (Hu Pancreas) Antibody

• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: CHYMO10-104.10, monoclonal
• mol wt: calculated mol wt 28 kDa
• species reactivity: human
• packaging: antibody small pack of 100 μg
• technique(s): ELISA: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• isotype: IgG1
• UniProt accession no.: P40313
• shipped in: dry ice
• storage temp.: -10 to -25°C
• target post-translational modification: unmodified

Description

General description

Chymotrypsin is a serine endopeptidase that is synthesized in the pancreatic acinar cells as chymotrypsinogen that is cleaved by trypsin to generate active chymotrypsin. Activation of chymotrypsinogen involves proteolytic cleavage at two sites. The resultant three chains are held together by five disulfide bonds. Chymotrypsin is reported to preferentially cleave peptide amide bonds where the carboxyl side of the amide bond (the P1 position) is a large hydrophobic amino acid (tyrosine, tryptophan, and phenylalanine). The aromatic ring in their sidechain that fits into a ′hydrophobic pocket′ (the S1 position) of the enzyme. Two predominant forms of chymotrypsin have been described and are designated as chymotrypsin A and B. They display high degree of homology but have significantly different proteolytic characteristics. The active site residues in chymotrypsin are far apart in the primary sequence but are brought together in a crevice formed between the two protein domains.

Specificity

Clone CHYMO10-104.10 is a mouse monoclonal antibody that detects human Chymotrypsin.

Immunogen

Purified human pancreatic chymotrypsin.

Application

Anti-Chymotrypsin (Hu Pancreas), clone CHYMO10-104.10, Cat. No. MAB1476-I, is a mouse monoclonal antibody that detects Trypsin and is tested for use in ELISA and Immunohistochemistry (Paraffin).

ELISA: (Dilution is assay dependent)

Immunohistochemistry: 1:1000.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Physical form

Purified mouse monoclonal antibody in buffer containing PBS, pH 7.4, containing no preservatives.

Storage and Stability

Maintain at -20°C in convenient aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 L or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable