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C1794

Sigma-Aldrich

Cellulase from Trichoderma sp.

greener alternative

BioReagent, suitable for plant cell culture, 3-10 units/mg solid

Synonym(s):

β-1,4-glucanase

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About This Item

CAS Number:
9012-54-8
Enzyme Commission number:
3.2.1.4 (BRENDA, IUBMB)
EC Number:
232-734-4
MDL number:
MFCD00081510
UNSPSC Code:
10171502
eCl@ss:
32160410
NACRES:
NA.72

biological source

fungus (Trichoderma sp.)

Quality Level

200

product line

BioReagent

form

powder

specific activity

3-10 units/mg solid

contains

maltodextrin

greener alternative product characteristics

Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

cell culture | plant: suitable

application(s)

agriculture

greener alternative category

, Enabling

storage temp.

2-8°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.

Application

Cellulase (β-1,4-glucanase) is a natural enzyme produced by the filamentous fungi Trichoderma viride which promotes the endohydrolysis of (1→4)-beta-D-glucosidic linkages in cellulose and lichenin. Cellulases are important for processing potential commercial products such as cellulosic ethanol.

Unit Definition

One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37 °C (2 hr incubation time).

substrate

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mari Aidemark et al.
BMC plant biology, 10, 274-274 (2010-12-16)
Alamethicin is a membrane-active peptide isolated from the beneficial root-colonising fungus Trichoderma viride. This peptide can insert into membranes to form voltage-dependent pores. We have previously shown that alamethicin efficiently permeabilises the plasma membrane, mitochondria and plastids of cultured plant
Rachel A Levin et al.
The Journal of eukaryotic microbiology, 64(5), 588-597 (2017-01-26)
Dinoflagellates within the genus Symbiodinium are photosymbionts of many tropical reef invertebrates, including corals, making them central to the health of coral reefs. Symbiodinium have therefore gained significant research attention, though studies have been constrained by technical limitations. In particular
Binay Bhushan Panda et al.
BMC plant biology, 18(1), 89-89 (2018-05-23)
Poor filling of grains in the basal spikelets of large size panicles bearing numerous spikelets has been a major limitation in attempts to increase the rice production to feed the world's increasing population. Considering that biotechnological intervention could play important
Jin Zhou et al.
Bioresource technology, 100(2), 819-825 (2008-09-06)
To improve the enzymatic hydrolytic efficiency and reduce production cost, a statistically designed experimental approach was used to optimize the composition of cellulase mixture so as to maximize the amount of glucose produced from steam-exploded corn stover (SECS). Using seven
Jit Ern Chen et al.
PloS one, 14(2), e0211936-e0211936 (2019-02-20)
Modern transformation and genome editing techniques have shown great success across a broad variety of organisms. However, no study of successfully applied genome editing has been reported in a dinoflagellate despite the first genetic transformation of Symbiodinium being published about
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Protocols

Enzymatic Assay of Cellulase

To standardize an enzymatic assay procedure of cellulase.

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