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  • Inhibition of the mitochondrial calcium uniporter prevents IL-13 and allergen-mediated airway epithelial apoptosis and loss of barrier function.

Inhibition of the mitochondrial calcium uniporter prevents IL-13 and allergen-mediated airway epithelial apoptosis and loss of barrier function.

Experimental cell research (2017-12-12)
Sara C Sebag, Olha M Koval, John D Paschke, Christopher J Winters, Alejandro P Comellas, Isabella M Grumbach
ABSTRACT

Mitochondria are increasingly recognized as key mediators of acute cellular stress responses in asthma. However, the distinct roles of regulators of mitochondrial physiology on allergic asthma phenotypes are currently unknown. The mitochondrial Ca

MATERIALS
Product Number
Brand
Product Description

Roche
In Situ Cell Death Detection Kit, TMR red, sufficient for ≤50 tests
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Tetramethylrhodamine methyl ester perchlorate, ≥95%
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Thapsigargin, ≥98% (HPLC), solid film
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Anti-ZO-1 Antibody, from rabbit, purified by affinity chromatography
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o-Cresolphthalein, indicator grade
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Tetramethylrhodamine isothiocyanate–Dextran, average mol wt 4,400
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Albumin from chicken egg white, lyophilized powder, ≥98% (agarose gel electrophoresis)
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Albumin from chicken egg white, lyophilized powder