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  • Novel method utilizing microbial treatment for cleaner production of diosgenin from Dioscorea zingiberensis C.H. Wright (DZW).

Novel method utilizing microbial treatment for cleaner production of diosgenin from Dioscorea zingiberensis C.H. Wright (DZW).

Bioresource technology (2013-08-27)
Mi Wei, Yun Bai, Mingzhang Ao, Wenwen Jin, Panpan Yu, Min Zhu, Longjiang Yu
ABSTRACT

A novel method utilizing microbial treatment for cleaner production of diosgenin from Dioscorea zingiberensis C.H. Wright (DZW) was presented. A new Bacillus pumilus HR19, which has the great ability to secrete pectinase, was screened and applied in the microbial treatment. Low-pressure steam expansion pretreatment (LSEP) was employed in advance to assist microbial treatment efficiently in releasing saponins, which are the precursors of diosgenin. Compared with the traditional process of acid hydrolysis, this novel process reduced the consumptions of water, acid and organic solvent by more than 92.5%, 97.0%, 97.0%, respectively, while simultaneously increasing the diosgenin yield by 6.21%. In addition, the microbial treatment was more efficient than enzymatic treatment, which arised from that microorganisms could be induced to secrete related enzymes by the compositions of DZW and relieve product inhibition by utilizing enzyme hydrolysates.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Pectolyase from Aspergillus japonicus, lyophilized powder, ≥0.3 units/mg solid
Sigma-Aldrich
Pectolyase from Aspergillus japonicus, suitable for plant cell culture, lyophilized powder
Sigma-Aldrich
Pectinase from Aspergillus niger, BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5 units/mg protein (Lowry)
Sigma-Aldrich
Pectinase from Rhizopus sp., powder, 400-800 units/g solid
Sigma-Aldrich
Pectinase from Aspergillus niger, powder, slightly beige, >1 U/mg