Skip to Content
Merck
All Photos(1)

Documents

C8571

Sigma-Aldrich

Cathepsin B from human liver

buffered aqueous solution, ≥1,500 units/mg protein (E1%/280)

Synonym(s):

Cathepsin B1

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
9047-22-7
Enzyme Commission number:
3.4.22.1 (BRENDA, IUBMB)
MDL number:
MFCD00130748
UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human liver

Quality Level

200

form

buffered aqueous solution

specific activity

≥1,500 units/mg protein (E1%/280)

UniProt accession no.

P07858

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CTSB(1508)

General description

Cathepsin B gene is mapped to human chromosome 8p23.1. It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence. It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal.

Application

Cathepsin B from human liver has been used:
  • as a positive control in the screening Cathepsin B inhibitor assay
  • in wall extension (expansin activity) assay
  • in microinjection human foreskin fibroblasts to test its effect on apoptosis induction

Biochem/physiol Actions

Cathepsin B from human liver is highly expressed in the esophageal adenocarcinoma (EAC). It reduces hepatic inflammation and fibrogenesis and may serve as a potential candidate for treating liver diseases.
Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Packaging

Package size based on protein content

Unit Definition

One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.

Physical form

Solution in 50 mM sodium acetate, pH 5.0, with 1 mM EDTA.

inhibitor

Product No.
Description
Pricing

related product

Product No.
Description
Pricing

substrate

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Prevention of free fatty acid-induced hepatic lipotoxicity by 18beta-glycyrrhetinic acid through lysosomal and mitochondrial pathways
Wu X, et al.
Hepatology, 47(6), 1905-1915 (2008)
Amino acid sequence of human liver cathepsin B
Ritonja A, et al.
Febs Letters, 181(1), 169-172 (1985)
Genome-wide catalogue of chromosomal aberrations in barrett's esophagus and esophageal adenocarcinoma: a high-density single nucleotide polymorphism array analysis
Gu J, et al.
Cancer Prevention Research (Philadelphia, Pa.), 3(9), 1176-1186 (2010)
The refined 2.15 AX-ray crystal structure of human liver cathepsin B: the structural basis for its specificity.
Musil DJ, et al.
The Embo Journal, 10(9), 2321-2330 (1991)
Cathepsin B inactivation attenuates hepatic injury and fibrosis during cholestasis
Canbay A, et al.
The Journal of Clinical Investigation, 112(2), 152-159 (2003)
View All Related Papers

Articles

Enzymatic Assay of Cathepsin B

This procedure applies to all products that have a specification for Cathepsin B activity determined by the liberation of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service