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76258

Sigma-Aldrich

Paraplast Plus®

suitable for electron microscopy, pellets (fast-melting)

Synonym(s):

Paraffin – polyisobutylene mixture

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12171500
NACRES:
NA.47

form

pellets (fast-melting)

technique(s)

electron microscopy: suitable

thickness

≤1 μm , short ribbons
≤2 μm , sectioning: continuous ribbons

mp

56-57 °C

application(s)

hematology
histology

storage temp.

2-8°C

General description

regular Paraplast with approx. 0.8% DMSO added for enhanced ease of infiltration and sectioning

Application

Paraffin polyisobutylene mixture is a tissue embedding medium composed of highly purified paraffin and polyisobutylene and a low level of dimethyl sulfoxide (DMSO) to enhance the rate of tissue penetration. Paraffin-polyisobutylene (76258) supports thin wrinkle-free sectioning down to 4 microM with more flexible and continuous ribbons.

Legal Information

Paraplast Plus is a registered trademark of Leica Biosystems

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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H O Goyal et al.
The Anatomical record, 250(2), 164-171 (1998-03-07)
Since androgens and/or estrogens must bind with specific receptors in order to elicit a response at the target organ(s), it is important to understand factors that regulate expression of androgen receptors (AR) and estrogen receptors (ER). Hence, the objective of
[Paraplast-plus, a tissue embedding medium that enables sections to stick to the slides without adhesives (author's transl)].
D Arn et al.
Mikroskopie, 31(3-4), 99-106 (1975-06-01)
H O Goyal et al.
The Anatomical record, 249(1), 54-62 (1997-09-19)
Because of the significance of androgens and estrogens in prenatal and postanatal differentiation of the testis and excurrent ducts, it is important to understand the developmental pattern of androgen receptor (AR) and estrogen receptor (ER) in these organs. Tissues from
Jing Qi et al.
Plant physiology, 147(4), 1947-1959 (2008-06-20)
The size and shape of the plant leaf is an important agronomic trait. To understand the molecular mechanism governing plant leaf shape, we characterized a classic rice (Oryza sativa) dwarf mutant named narrow leaf1 (nal1), which exhibits a characteristic phenotype
R S Liem et al.
Acta anatomica, 132(2), 164-168 (1988-01-01)
A method is described for the histochemical detection of horseradish peroxidase in Paraplast Plus embedded brain sections. The procedure uses 150-micron-thick Vibratome-cut slices of glutaraldehyde-paraformaldehyde-fixed brain tissue. Tetramethylbenzidine stabilized by diaminobenzidine/cobalt/H2O2 is used as chromogen. The Vibratome-cut slices are dehydrated

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