{"metaDataObj":{"isNoIndex":false,"isNoFollow":false,"keywords":["Technical Article","Protein purification"],"title":"Troubleshooting Methods for Purification of MBP-Tagged Recombinant Proteins","seoSchemas":[{"@context":"https://schema.org","@type":"BreadcrumbList","itemListElement":[{"@type":"ListItem","position":1,"name":"Protein Purification","item":"%%HOMEPAGE_URL%%/DE/en/applications/protein-biology/protein-purification"},{"@type":"ListItem","position":2,"name":"Troubleshooting Methods for Purification of MBP-Tagged Recombinant Proteins"}]}],"description":"Troubleshooting of methods for purification of MBP-tagged recombinant proteins using Cytiva products.","pageType":"Technical Article","ogImageMetaInfo":[{"ogTitle":"Troubleshooting Methods for Purification of MBP-Tagged Recombinant Proteins","ogType":"website","ogImage":"https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/social-media/vibrant-m/vibrant-m.jpg","ogUrl":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins","ogDescription":"Troubleshooting of methods for purification of MBP-tagged recombinant proteins using Cytiva products."}]},"designPath":"/libs/settings/wcm/designs/default","cssClassNames":"page basicpage","title":"Troubleshooting Methods for Purification of MBP-Tagged Recombinant Proteins","templateName":"technical-article---protocol-page-template","lastModifiedDate":1621202722217,"language":"en",":itemsOrder":["root"],":items":{"root":{"columnClassNames":{"rightrailresponsivecontainer":"aem-GridColumn aem-GridColumn--default--3 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","footer":"aem-GridColumn aem-GridColumn--default--12","utilityresponsivecontainer":"aem-GridColumn aem-GridColumn--default--3 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","bodyresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","topresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9 aem-GridColumn--offset--default--0 aem-GridColumn--default--none"},"gridClassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columnCount":12,"allowedComponents":{"applicable":false,"components":[{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/aboutus","title":"About Us Text"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/accordion","title":"Accordion"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/audio","title":"Audio"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/author","title":"Author"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/breadcrumb","title":"Breadcrumbs - Page Path"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/button","title":"Button"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/card","title":"Card"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/carousel","title":"Carousel"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/categorysearchresults","title":"Category Search Results"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/cftext","title":"CF Text"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/contact","title":"Contact Component"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/container","title":"Container component"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/dealerlicense","title":"Dealer License"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/dynamicproducttable","title":"Dynamic Product Table"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/event-sidebar","title":"Event Sidebar"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/eventlisting","title":"Event Listing"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/experiencefragment","title":"Experience Fragment"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/featuretiles","title":"Feature Tiles"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/footer","title":"Footer"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/formAssembly","title":"Form Assembly"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/gatedbutton","title":"Gated Button"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/gatedcontent","title":"Gated Content"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/globalfooter","title":"Global Footer"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/header","title":"Header"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/herocomponent","title":"Hero Component"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/workflows/horizontal","title":"Workflow Horizontal"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/horizontaltab","title":"Tabbed Horizontal Navigation"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/image","title":"Image"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/leftnavigation","title":"Navigation Model"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/locationmap","title":"Map"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/pressreleasegrid","title":"Related Press releases"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/pressreleaselisting","title":"Press release listing"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/presstext","title":"Region Text"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/primarycontent","title":"Primary Content"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/printsocialsharing","title":"Print Social sharing"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/productRecommendationCarousel","title":"Product Recommendation Carousel"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/promobanner","title":"Promo Banner"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/rawhtml","title":"Raw HTML"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/recommendedSearch","title":"Recommended Search Term"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/reference","title":"Reference"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/relatedContentCarousel","title":"Related Content Carousel"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/relatedcontentgrid","title":"Related Content Grid"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/relatedcontentlinks","title":"Related Content Links"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/relatedproductresources","title":"Related Product Resources"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/relatedproducts","title":"Related Products Carousel"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/relatedproductsrightrail","title":"Related Products Right Rail"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/relatedwebinar","title":"Related Webinar"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/sectiondivider","title":"Section Divider"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/sitecontentsearch","title":"Site Content Search"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/sitemap","title":"Site Map"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/speakerpage","title":"Speaker Page"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/speakers-list","title":"Speakers List"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/table","title":"Static Table"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/tagbasedbreadcrumb","title":"Breadcrumbs - Tag Based"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/targetcontainer","title":"Target Container component"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/targetescapehatch","title":"Target Escape Hatch"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/text","title":"Text"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/textbanner","title":"Text Banner"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/title","title":"Title"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/topPagesCarousel","title":"Top Pages Carousel"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/topnavigation","title":"Top Navigation"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/upcomingeventsgrid","title":"Upcoming Events"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/workflows/vertical","title":"Workflow Vertical"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/video","title":"Video"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/webinarlisting","title":"Webinar listing"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/webinarsidebar","title":"Webinar Sidebar"},{"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/cms-commons/components/content/worldwideofficelisting","title":"Worldwide Office Listing"}]},":itemsOrder":["topresponsivecontainer","utilityresponsivecontainer","bodyresponsivecontainer","rightrailresponsivecontainer","footer"],":items":{"topresponsivecontainer":{"columnClassNames":{"tagbasedbreadcrumb":"aem-GridColumn aem-GridColumn--default--9","cq:responsive":"aem-GridColumn aem-GridColumn--default--9","title":"aem-GridColumn aem-GridColumn--default--9"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--9","columns":12,"halfGatedContent":false,"items":[{"url":null,"title":null,"description":null,"lastModified":1621202721757,"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/topresponsivecontainer/tagbasedbreadcrumb","name":"tagbasedbreadcrumb"},{"url":null,"title":null,"description":null,"lastModified":1621202721783,"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/topresponsivecontainer/title","name":"title"}],"allowedComponents":{"applicable":false,"components":[]},":itemsOrder":["tagbasedbreadcrumb","title"],":items":{"tagbasedbreadcrumb":{"items":[{"url":"/DE/en","title":"Home","titleEng":"Home","active":false,"nonClickable":false},{"url":"/DE/en/applications/protein-biology/protein-purification","title":"Protein Purification","titleEng":"Protein Purification","active":false,"nonClickable":false},{"url":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins","title":"Troubleshooting Methods for Purification of MBP-Tagged Recombinant Proteins","titleEng":"Troubleshooting Methods for Purification of MBP-Tagged Recombinant Proteins","active":true,"nonClickable":true}],":type":"cms-commons/components/content/tagbasedbreadcrumb"},"title":{"anchorId":null,"openNewTab":false,"linkURL":null,"text":"Troubleshooting Methods for Purification of MBP-Tagged Recombinant Proteins","type":"h1",":type":"cms-commons/components/content/title"}},"authenticatedUserBGColor":"","unauthenticatedUserBGColor":"",":type":"cms-commons/components/content/container"},"utilityresponsivecontainer":{"columnClassNames":{"cq:responsive":"aem-GridColumn aem-GridColumn--default--3"},"cssClassName":"cms-container--top","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--3","columns":12,"halfGatedContent":false,"items":[],"allowedComponents":{"applicable":false,"components":[]},":itemsOrder":[],":items":{},"authenticatedUserBGColor":"","unauthenticatedUserBGColor":"",":type":"cms-commons/components/content/container"},"bodyresponsivecontainer":{"columnClassNames":{"reference":"aem-GridColumn aem-GridColumn--default--9","dynamicproducttable":"aem-GridColumn aem-GridColumn--default--9","middleresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9","topcontainer":"aem-GridColumn aem-GridColumn--default--9","cq:responsive":"aem-GridColumn aem-GridColumn--default--9"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--9","columns":12,"halfGatedContent":false,"items":[{"url":null,"title":null,"description":null,"lastModified":1621202721967,"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/bodyresponsivecontainer/topcontainer","name":"topcontainer"},{"url":null,"title":null,"description":null,"lastModified":1621202722017,"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/bodyresponsivecontainer/dynamicproducttable","name":"dynamicproducttable"},{"url":null,"title":null,"description":null,"lastModified":1621202722026,"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/bodyresponsivecontainer/middleresponsivecontainer","name":"middleresponsivecontainer"},{"url":null,"title":null,"description":null,"lastModified":1621202722035,"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/bodyresponsivecontainer/reference","name":"reference"}],"allowedComponents":{"applicable":false,"components":[]},":itemsOrder":["topcontainer","dynamicproducttable","middleresponsivecontainer","reference"],":items":{"topcontainer":{"columnClassNames":{"table":"aem-GridColumn aem-GridColumn--default--12"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"halfGatedContent":false,"items":[{"url":null,"title":null,"description":null,"lastModified":1621202722001,"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/bodyresponsivecontainer/topcontainer/table","name":"table"}],"allowedComponents":{"applicable":false,"components":[]},":itemsOrder":["table"],":items":{"table":{"anchorId":null,"enableSort":false,"verticalTable":false,"headline":null,"title":null,"caption":null,"table":"\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n

Problem	Possible cause	Solution
Increased back pressure	High viscosity of solutions.	Use lower ﬂow rates and/or dilute the sample.
Insufﬁcient cell disruption.	Increase the efﬁciency of the mechanical cell disruption, e.g., increase sonication time. (Keep the sample on ice during sonication to avoid frothing and overheating as this may denature the target protein. Over-sonication can also lead to co-puriﬁcation of host proteins with the target protein). Increase dilution of the cell paste before mechanical lysis, or dilute after lysis to reduce viscosity. Decrease ﬂow rate during sample loading.
Freezing/thawing of the unclariﬁed lysate has increased precipitation and aggregation.	Centrifuge or pass through a 0.22 or 0.45 µm ﬁlter before application on the column.
Column has clogged	Top ﬁlter is clogged.	Change top ﬁlter. If using an MBPTrap column, replace the column.
Cell debris in the sample may have clogged the column.	Clean the column according to Appendix 3 (Characteristics of Dextrin Sepharose High Performance products). Centrifuge and/or ﬁlter the sample through a 0.22 µm or a 0.45 µm ﬁlter or otherwise optimize sample pretreatment before loading the next sample.
No or weak binding	Protein found in the ﬂowthrough.	Buffer/sample composition is not optimal; check the pH and composition of the sample and binding buffer. pH should in general be above pH 7.
Factors in the crude extract interfere with binding.	Include glucose in the growth medium to suppress amylase expression.
MBP-tag is not present.	Use protease-deﬁcient E. coli expression strains. Add protease inhibitors during cell lysis.
MBP-tag is not accessible.	Fuse the MBP-tag with the other protein terminus. Use another linker.
Protein has precipitated in the column due to high protein concentration.	Clean the column according to instructions in to Appendix 3 (Characteristics of Dextrin Sepharose High Performance products). In the following run decrease the amount of sample, or decrease protein concentration by eluting with a linear gradient instead of step-wise elution. Try detergents or change the NaCl concentration. If an MBPTrap HP 1 ml column has been used, change to the larger MBPTrap HP 5 ml. This will reduce the ﬁnal concentration, provided that the same amount of sample is applied. For quick scale-up, connect two or more columns in series by screwing the end of one column into the top of the next. Note, however, that connecting columns in series will increase back pressure.
Contaminating proteins	Contaminants are short forms of the tagged protein.	Use protease-deﬁcient E. coli expression strains. Add protease inhibitors after cell lysis. Fuse the MBP-tag with the other protein terminus. Check for the presence of internal translation initiation starts (for C-terminal MBP-tag) or premature termination sites (for N-terminal MBP-tag). Use EDTA in the sample and buffers. Keep the sample cold.
Contaminants are covalently linked to the recombinant protein via disulﬁde bonds.	Add reducing agents to all buffers for cell lysis and puriﬁcation. Note that the yield may decrease.
Contaminants are non-covalently linked to the recombinant protein.	Increase ionic strength in all buffers for cell lysis and puriﬁcation (up to 1 M NaCl) or add mild detergents, 0.1% Tween, 0.1% CHAPS). Be careful since the binding of MBP to dextrin may be affected by the addition of non-ionic detergents.
Unwanted air bubble formation	Unclariﬁed lysates may increase air bubble formation during puriﬁcation.	Attaching a ﬂow restrictor in the chromatography system can prevent this. If a ﬂow restrictor is attached, it is important to change the pressure limit to adjust for the extra pressure from the ﬂow restrictor. Do not exceed the pressure limit for the column on the ÄKTA system.
Air bubbles may form due to decreased air solubility when columns stored at 2 °C to 8 °C are used immediately at room temperature.	Let the columns adapt to room temperature for some minutes before using them.

\n",":type":"cms-commons/components/content/table"}},"authenticatedUserBGColor":"","unauthenticatedUserBGColor":"",":type":"cms-commons/components/content/container"},"dynamicproducttable":{"anchorId":null,"productsArray":["GE28-9187-80 Sigma (SIGMA)","GE28-9187-79 Sigma (SIGMA)","GE28-9187-78 Sigma (SIGMA)","GE29-0486-41 Sigma (SIGMA)"],"isShowImage":false,"maxRows":20,"products":[{"productKey":"GE28918780","brandKey":"SIGMA"},{"productKey":"GE28918779","brandKey":"SIGMA"},{"productKey":"GE28918778","brandKey":"SIGMA"},{"productKey":"GE29048641","brandKey":"SIGMA"}],"tableTitle":"Materials",":type":"cms-commons/components/content/dynamicproducttable"},"middleresponsivecontainer":{"columnClassNames":{},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"halfGatedContent":false,"items":[],"allowedComponents":{"applicable":false,"components":[]},":itemsOrder":[],":items":{},"authenticatedUserBGColor":"","unauthenticatedUserBGColor":"",":type":"cms-commons/components/content/container"},"reference":{"headline":null,"anchorId":null,"startNumber":null,"references":null,":type":"cms-commons/components/content/reference"}},"authenticatedUserBGColor":"","unauthenticatedUserBGColor":"",":type":"cms-commons/components/content/container"},"rightrailresponsivecontainer":{"columnClassNames":{"relatedcontentlinks":"aem-GridColumn aem-GridColumn--default--3","cq:responsive":"aem-GridColumn aem-GridColumn--default--3"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--3","columns":12,"halfGatedContent":false,"items":[{"url":null,"title":null,"description":null,"lastModified":1621202722092,"path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins/jcr:content/root/rightrailresponsivecontainer/relatedcontentlinks","name":"relatedcontentlinks"}],"allowedComponents":{"applicable":false,"components":[]},":itemsOrder":["relatedcontentlinks"],":items":{"relatedcontentlinks":{"contentType":"sigma-aldrich:content-type/page-type/technical-article","linksNumber":8,"anchorId":null,"rightRail":false,"ctaLink":"/DE/en/search/facet-search?focus=sitecontent&term=facet-search&facet=facet_content_type%3ATechnical+Article&facet=facet_applications%3AProtein+purification","title":"Related Articles","tags":["sigma-aldrich:applications/protein-biology/protein-purification"],"ctaTitle":null,"categories":[{"categoryTitle":"Extract-N-Amp™ Product Guide","categoryUrl":"/DE/en/technical-documents/protocol/genomics/pcr/extract-n-amp-product-guide","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography","categoryUrl":"/DE/en/technical-documents/protocol/protein-biology/protein-purification/column-packing-and-preparation2","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Maintenance of Multimodal Chromatography Media and Storage Conditions","categoryUrl":"/DE/en/technical-documents/protocol/protein-biology/protein-purification/maintenance-of-media-and-storage-conditions","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Manual Purification of Histidine-Tagged Proteins using His GraviTrap™ TALON® Columns","categoryUrl":"/DE/en/technical-documents/protocol/protein-biology/protein-purification/manual-purification-using-his-gravitrap-talon","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Purification of Thiol-Containing Substances By Covalent Chromatography","categoryUrl":"/DE/en/technical-documents/technical-article/analytical-chemistry/purification/covalent-chromatography","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Immobilized Metal Chelate Affinity Chromatography (IMAC)","categoryUrl":"/DE/en/technical-documents/technical-article/analytical-chemistry/purification/metal-chelate-afnity-chromatography","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Desalting and Buffer Exchange for Affinity Chromatography of Antibodies","categoryUrl":"/DE/en/technical-documents/technical-article/protein-biology/protein-concentration-and-buffer-exchange/desalting-buffer-exchange","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Salt Selection and Buffer Preparation","categoryUrl":"/DE/en/technical-documents/technical-article/protein-biology/protein-concentration-and-buffer-exchange/salt-selection-and-buffer-preparation","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false}],":type":"cms-commons/components/content/relatedcontentlinks"}},"authenticatedUserBGColor":"","unauthenticatedUserBGColor":"",":type":"cms-commons/components/content/container"},"footer":{"richText":false,":type":"cms-commons/components/content/footer"}},":type":"wcm/foundation/components/responsivegrid"}},":hierarchyType":"page",":type":"cms-commons/components/structure/page",":path":"/DE/en/technical-documents/technical-article/protein-biology/protein-purification/troubleshooting-purification-of-mbp-tagged-recombinant-proteins"}