Custom DNA Oligos: Standard DNA Synthesis

DNA Oligo Specifications

Synthesis Scales	0.025, 0.05, 0.2, 1.0, 10, and 15µmole mg - gram quantity manufacturing available upon request
Purification	Desalt, Cartridge, HPLC, PAGE See Oligonucleotide Purification Guidelines
Length	Up to 120 bases, consult technical support for feasibility of longer oligos up to 180 bases
Backbone	Phosphodiester / Phosphorothioate
Format	Dry or In Solution (normalized)
Modifications	Over 200 available, including dyes (fluorescent and non-fluorescent)
Packaging	2 mL - 50 mL Tubes, Microtiter Plates
Quality Controls	MALDI-TOF MS, Electrospray Ionization MS, OD by UV Spectroscopy
Technical Datasheet & Labels	Includes yield, Tm (melting temperature), MW, and µg/OD Duplicate set of labels with each order
Additional Services	Concentration adjustment Aliquoting into tubes or well plates MSQC traces available upon request

DNA Oligos in Plates

We can format your oligos to best suit your application as well as your automation and information requirements.

Custom specifications can include:

• 96 or 384 well plates: Various plate types available
• Concentration normalization options with fixed or variable volumes
• Dried or in solution (water or TE)

In addition to the common formats listed above, other options are available including:

• 1-D or 2-D Barcoding
• Customer Specified Plate and Buffers
• Daughter Plates (Copies)
• Forward & Reverse Primers in a Single Well

Quality

Each shipment includes data sheets for import into customers’ information systems containing well location, sequence, oligo name, lot number, MW, µg/OD, length, T_m, concentration and volume (in solution).

Additional quality control measures are in place to verify accuracy for custom formats. These measures may include:

• Plate orientation and well position verification by mass spectrometry
• Mixed Primers, i.e. Forward and Reverse in a single well, verified by mass spectrometry
• Verification for machine readable barcodes (1D and 2D)
• Visual inspection of all master and daughter plates prior to shipment

Learn more about our quality control and quality assurance.

Concentration Verification and Plate Identity

We employ a variety of liquid-handling automation systems to normalize the oligo concentration. A multi-step normalization process ensures the concentrations are within a narrow range. At the end of the process, a final absorbance reading (measured at 260 nm) ensures that every oligo is at the desired concentration.

Custom Oligo Pools

We can provide custom oligo pools to your specification and required format. Our custom formatting capabilities have been optimized to provide complex formulations containing hundreds of oligos in a single pool.

Oligos pools can be provided normalized to a specific amount or concentration in water or buffer and supplied in liquid or dry format. Multiple aliquots of the oligo pool can be provided upon request. In addition to standard mixes, we specialize in preparing complex formulations containing varying amounts per oligo within the pool. Simple oligo mixes can be provided in plates or tubes, complex formulations containing a large number of oligos will be provided in the appropriate tube based on the pool volume or the customers required format.

Please contact dnaoligos@milliporesigma.com to request a quote.

Minimum Yield Guarantees

Non-Modified Oligos (O.D./µg)*
Scale (µmole)	Desalt	Cartridge	HPLC	PAGE
0.025	3/90	NA	NA	NA
0.05	5/150	1/30	1/30	0.5/15
0.2	12/360	3/90	2.5/75	1/30
1.0	40/1,200	12/360	13/390	5/150
10	400/12,000	NA	130/3,900	NA
15	600/18,000	NA	190/5,700	NA

*Guarantees are for 20 mers or longer. Oligos shorter than 20 bases will have fewer ODs. Microgram amounts are calculated based on a 30 μg/OD value. If the μg/OD value is less than 30, the reported μg value will be proportionally less. Phosphorothioated yields are 70% of the above stated values.

Shipping Schedule

Scale (µmole)	Desalt	Cartridge	HPLC	PAGE
0.025	24 hours	NA	NA	NA
0.05	1-2 days	1-2 days	3-4 days	Inquire
0.2	1-2 days	1-2 days	3-4 days	Inquire
1.0	1-2 days	1-2 days	3-4 days	Inquire
Plates (non-modified)*	3-5 days	3-5 days	Inquire	Inquire
Greater than 1.0 µmole	10 days or less	10 days or less	10 days or less	Inquire
Large quantity	Inquire	Inquire	Inquire	Inquire
Post-synthesis modified	7-10 days	7-10 days	7-10 days	Inquire
All other modifications	5-7 days or less	5-7 days or less	5-7 days or less	Inquire

* Large projects will be placed on a delivery schedule based upon the customers project needs.