Gastrointestinal Organoid Biobank

Patient derived organoids (PDOs) are novel in vitro 3D cell models that preserve original tissue physiology and molecular pathology, thus representing a clinically-relevant alternative to traditional 2D cell lines and an effective tool to refine and reduce animal models. PDOs can be derived from adult patient biopsies or resected tissues containing native LGR5+ stem cell populations and cultured within an ECM-rich substrate using specialized organoid media such as L-WRN conditioned media (SCM105). Adult tissue derived organoids are phenotypically and genetically stable in long term culture, presenting more mature phenotypes compared to iPSC-derived organoids. Importantly, PDOs have shown to be able to predict patient clinical responses to chemotherapeutics.

We are now offering a comprehensive biobank of highly characterized tissue-derived human gastrointestinal organoids from normal and diseased patients. The intestinal organoid biobank contains over 50 highly characterized intestinal organoids from both normal and diseased patients derived from multiple regions of the digestive systems including small intestine (duodenum, ileum), stomach, rectum and colon.

3dGRO^™ organoids were derived utilizing HUB Organoid Technology. The purchaser of this product shall agree to HUB’s terms of use, which shall be separately acknowledged and accepted by such purchaser, prior to transfer of this product to purchaser.

Biobank Overview

• 58 Patient Derived Organoid Samples
• Multiple Genders, Age, and Sex
• Multiple Tissues/Diseases: Colon, Duodenum, Ilium, Stomach, Rectum, Cancer, Ulcerative Colitis, IBS
• RNA-Seq Available
• Low Passage and High Viability
• Cultured in Optimized 3dGRO^™ L-WRN Conditioned Media

Prep-78-C (SCC311)

Prep-87-C (SCC321)

Prep-81-C (SCC313)

14-881-CR (SCC310)

Figure 1. Patient-derived colon organoids from both normal (SCC311, SCC321, SCC313) and diseased patients (rectal adenocarcinoma, SCC310) cultured in L-WRN conditioned media (SCM105) for five passages.

Ordering Information for PDO Biobank

* Coming Soon ** Does not require HUB terms of use agreement prior to purchase.

ID	Catalog Number	Age	Sex	Organ	Disease Type or Normal
14-881-CR	SCC310	46	M	Colon (Rectum)	Adenocarcinoma (TP53 Pro151Ser, KRAS Gly12Asp, APC Pro1443fs) (RNA-seq)
Prep 78-C	SCC311	52	F	Colon	Normal (RNA-seq)
Prep 80-C	SCC312	29	F	Colon	Normal (RNA-seq)
Prep 81-C	SCC313	49	F	Colon	Normal (RNA-seq)
Prep 81-D	SCC314	49	F	Duodenum	Normal (RNA-seq)
Prep 83-C	SCC315	45	F	Colon	Normal (RNA-seq)
Prep 83-D	SCC316	45	F	Duodenum	Normal (RNA-seq)
Prep 84-C	SCC317	56	F	Colon	Normal (RNA-seq)
Prep 84-D	SCC318	56	F	Duodenum	Normal (RNA-seq)
Prep 85-C	SCC319	62	F	Colon	Normal (RNA-seq)
Prep 85-D	SCC320	62	F	Duodenum	Normal (RNA-seq)
Prep 87-C	SCC321	21	M	Colon	Normal (RNA-seq)
Prep 87-D	SCC322	21	M	Duodenum	Normal (RNA-seq)
Prep 88-C	SCC323	33	F	Colon	Normal (RNA-seq)
Prep 88-D	SCC324	33	F	Duodenum	Normal (RNA-seq)
Prep 89-C	SCC325	55	M	Colon	Normal (RNA-seq)
Prep 89-D	SCC326	55	M	Duodenum	Normal (RNA-seq)
18-1417-N	SCC327*	45	M	Colon	Normal (CRC tissue adjacent)
ht-131-C	SCC328*	58	F	Colon	Adenoma
ht-098-C	SCC329	70	F	Colon	Normal (RNA-seq)
ht-099-C	SCC330	59	F	Colon	Normal (RNA-seq)
ht-100-C	SCC331*	67	M	Colon	Normal
ht-101-C	SCC332	59	F	Colon	Normal (RNA-seq)
ht-101-D	SCC333*	59	F	Duodenum	Normal
ht-102-C	SCC334*	61	F	Colon	Normal
ht-103-C	SCC335	63	M	Colon	Normal
ht-104-C	SCC336*	51	M	Colon	Normal
ht-104-I	SCC337	51	M	Ileum	Normal (RNA-seq)
ht-105-C	SCC338*	19	M	Colon	Normal
ht-105-I	SCC339	19	M	Ileum	Normal (RNA-seq)
ht-118-C	SCC340	43	M	Colon	Normal
ht-119-C	SCC341*	53	F	Colon	Normal
ht-127-S	SCC342	52	F	Stomach	Normal
ht-128-S	SCC343	60	F	Stomach	Normal
ht-129-S	SCC344	60	M	Stomach	Normal
ht-130-S	SCC345	68	M	Stomach	Normal
ht-215-CD	SCC346*	59	F	Colon descending	Normal
ht-215-CT	SCC347*	59	F	Colon transcending	Normal
ht-215-D	SCC348*	59	F	Duodenum	Normal
ht-222-D	SCC349*	33	M	Duodenum	Normal
ht-180-D	SCC350	38	F	Duodenum	Normal
ht-185-D	SCC351	54	M	Duodenum	Normal
ht-188-D	SCC352*	50	F	Duodenum	Normal margin to UC
ht-205-CS	SCC353	33	F	Colon	Normal Margin to Crohns
ht-205-D	SCC354	33	F	Duodenum	Normal Margin to Crohns
ht-205-I	SCC355	33	F	Ileum	Normal Margin to Crohns
ht-213-CA	SCC356*	36	F	Colon ascending	Normal margin to UC
ht-213-CD	SCC357*	36	F	Colon descending	Normal margin to UC
ht-213-CT	SCC358*	36	F	Colon transcending	Normal margin to UC
ht-213-D	SCC359*	36	F	Duodenum	Normal margin to UC
ht-214-C	SCC359*	36	F	Duodenum	Normal margin to UC
ht-214-C	SCC360*	46	M	Colon J-pouch	Normal margin to UC
ht-214-SI	SCC361*	46	M	Small Intestine J-pouch	Normal margin to UC
ht-227-D	SCC362*	62	M	Duodenum	Normal margin to UC
ht-230-D	SCC363*	59	F	Duodenum	Normal margin to UC
ht-205-I	SCC364*	33	F	Ileum	UC
ht-206-C	SCC365	24	F	Colon	Crohns
ht-206-I	SCC366	24	F	Ileum	Crohns
ht-208-CR	SCC367	23	M	Colon (Rectum)	Crohns
ISO38	SCC501**	N/A	N/A	Sigmoid Colon	Colorectal Cancer (TP53 Pro151Ser, KRAS Gly12Asp, APC Pro1443fs)
ISO48	SCC502**	N/A	N/A	Caecum	Colorectal Cancer (PIK3CA ∆E542K, CTNNB1 ∆S33C)
ISO49	SCC503**	N/A	N/A	Lower Sigmoid Colon	Colorectal Cancer (APC ∆R1450*A1446Lfs*27, TP53 ∆R248Q, KRAS ∆G12D, PIK3CA ∆E542K, FBXW7 ∆R465C)
ISO50	SCC504**	N/A	N/A	Colon (Rectum)	Colorectal Cancer (APC ∆R232*E1286*, TP53 ∆R248Q", KRAS ∆G12D, SMAD4 ∆E526K, SMAD2 ∆S464*)
ISO57	SCC505**	N/A	N/A	Colon (Upper Rectum)	Colorectal Cancer (APC ∆Q978*, TP53 ∆R282W, KRAS ∆G13D)
ISO68	SCC506**	N/A	N/A	Sigmoid Colon	Colorectal Cancer (APC ∆Q1096*E1408*, TP53 ∆R248W, KRAS ∆G13D, SMAD4 ∆Q534*)
ISO72	SCC507**	N/A	N/A	Caecum	Colorectal Cancer (APC ∆Q1291*, KRAS ∆G12D, ERBB3 ∆A232V)

Fig 2: Immunocytochemical characterization of human colon organoids (Colon-87, SCC321). Human colon PDOs are positive for colon-specific markers: CA II, CA IV and Mucin5B, posterior hindgut marker: CDX2, stem cell markers: Lgr5 and Sca1 and epithelial markers: TPH-1 and E-Cad.

Fig 3: Heatmap of top 30 differentially expressed genes

Fig 4: Gene expression differences of human iPSCs, iPSC derived colon organoids vs. adult patient derived tissue organoids.

Fig 5: Adult tissue derived colon (SCC321, Colon-87) and duodenum (SCC320, DUO-85) organoids express SARS-CoV-2 related proteins ACE2 and TMPRSS2.