Purospher™ STAR HPLC and UHPLC Columns

U/HPLC Separations with Excellent Peak Symmetry

Purospher™ STAR HPLC and UHPLC columns are based on 99.9999 % ultra-pure fully porous silica (Type B). They are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Thanks to these features, Purospher™ STAR HPLC columns allow high-throughput applications, and allow maximum flexibility to use the best chromatographic conditions for any separation including reversed phase.

Features and Benefits

• Ultra-pure silica (99.999%) for excellent peak symmetry
• High separation efficiency
• Reproducible results from run-to-run and batch-to-batch
• Best all-round performance according to Tanaka
• Outstanding pH stability from pH 1.5 to 10.5
• No phase collapse when using highly aqueous mobile phases
• Good suitability for LC-MS applications

Purospher™ STAR HPLC Column Properties and Use

Modification	USP	Particle size	Pore Size (Å)	Spec. Surface Area (m2/g)	C %	pH Stability	Use
RP-18 endcapped	L1	5 µm 3 µm 2 µm	120	330	17	1.5 – 10.5	Best in Class C18 column for excellent peak symmetry, performance and pH stability
RP-8 endcapped	L7	5 µm 3 µm 2 µm	120	330	10.5	1.5 – 10.5	Less hydrophobic compounds, faster retention of very hydrophobic compounds
Phenyl	L11	5 µm 3 µm 2 µm	120	330	12.5	1.5 – 10.5	Enhanced selectivity for the separation of aromatic compounds due to Pi-Pi Interactions
NH2	L8	5 µm	120	330	3.5	2 – 7.5	Separation of carbohydrates and polar compounds with normal-phase or HILIC chromatography
Si	L3	5 µm	120	330	-	2 – 7.5	Separation of polar compounds with normal-phase or HILIC (Si) chromatography

Figure 1. LiChroCART

Purospher™ STAR in stainless steel LiChroCART^® cartridges

The LiChroCART^® columns (75, 125, 150 and 250 mm length) in the list above (2, 3, 4 and 4.6 mm i.d.) require part number 1.51486.0001 manu-CART^® cartridge column holder, which can be used to hold one cartridge column with or without a 4-4 mm guard column. LiChroCART^® columns 250-10 mm require part number 1.51419.0001 manu-CART^® 10.

The short LiChroCART^® columns (30 and 55 mm length) can be ordered as a set including the corresponding cartridge holder and one cartridge, or as a pack of 3 cartridges without cartridge holder. The separate part numbers for the cartridge are as follows: 1.50227.0001 LiChroCART^® cartridge holder for 30 mm cartridge and 1.50226.0001 LiChroCART^® cartridge holder for 55 mm cartridge

LiChroCART^® Cartridge 2 mm i.d.

Modification	Particle size	30-2	55-2	100-2	125-2	150-2	250-2
RP-18 endcapped	3 μm	1.50238**	1.50241**
RP-18 endcapped Set*	3 μm	1.50241**	1.50240*
RP-18 endcapped	5 μm	1.50229	1.50234	1.50623	1.50255	1.50624	1.50256
RP-8 endcapped	3 μm	1.50229	1.50234
RP-8 endcapped	5 μm				1.50274		1.50275

LiChroCART^® Cartridge 3 mm i.d.

Modification	Particle size	30-3	55-3	100-3	125-3	150-3	250-3
RP-18 endcapped	3 μm	1.50233	1.50236
RP-18 endcapped	5 μm	1.50233	1.50236	1.50625	1.50253	1.50626	1.50254
RP-8 endcapped	5 μm				1.50038		1.50237

LiChroCART^® Cartridge 4 mm i.d.

Modification	Particle size	4-4 (10 guard columns)	30. Apr	55-4	75-4	125-4	250-4
RP-18 endcapped	3 μm		1.50225**	1.50231**	1.51460
RP-18 endcapped Set*	3 μm		1.50239*	1.50242*
RP-18 endcapped	5 μm	1.50250	1.50302	1.50228		1.50251	1.50252
RP-8 endcapped	5 μm	1.50270				1.50271	1.50272
NH2	5 μm	1.50267				1.50244	1.50245
Si	5 μm	1.50249				1.50268	1.50269

LiChroCART^® Cartridge 4.6 mm i.d.

Modification	Particle size	4-4 (10 guard columns)	100-4.6	150-4.6	250-4.6
RP-18 endcapped	3 μm		1.51448
RP-18 endcapped	5 μm	1.50250	1.50627	1.50358	1.50359
RP-8 endcapped	5 μm	1.50270		1.50031	1.50032
Phenyl	5 μm			1.51922	1.51921
NH2	5 μm	1.50267		1.50247	1.50248
Si	5 μm	1.50249		1.50356	1.50357

LiChroCART^® Cartridge 10 mm i.d.

Modification	Particle size	10-10 (guard column)	75-10	100-10	125-10	150-10	250-10
RP-18 endcapped	5 μm	1.50178	1.51449	1.51445	1.51443	1.51444	1.50257
RP-8 endcapped	5 μm						1.50276

* One set contains: 1 LiChroCART^® cartridge and one holder

Purospher™ STAR in Hibar^® RT columns

The Hibar^® RT columns are complete with endfittings. When using a guard column with a Hibar^® RT column, we recommend part number 1.51487.0001 guard column cartridge holder for 4-4 mm guard column cartridges LiChroCART^®.

Figure 2. Purospher™ STAR in Hibar® RT columns

Hibar^® RT Column 2 mm i.d.

Modification	Particle size	50-2	100-2	125-2	150-2	250-2
RP-18 endcapped	5 μm	1.50593	1.50595	1.50596	1.50597	1.50598

Hibar^® RT Column 3 mm i.d.

Modification	>Particle size	50-3	100-3	125-3	150-3	250-3
RP-18 endcapped	3 μm	1.50393	1.50398	1.50413	1.50414	1.50427
RP-18 endcapped	5 μm	1.50607	1.50612	1.50615	1.50617	1.50620
RP-8 endcapped	3 μm				1.50750
RP-8 endcapped	5 μm				1.50644
Phenyl	3 μm				1.50631
Phenyl	5 μm				1.51920

Hibar^® RT Column 4 mm i.d.

Modification	Particle size	50-4	125-4	250-4
RP-18 endcapped	3 μm	1.50428	1.50431	1.50468
RP-18 endcapped	5 μm	1.50621	1.50036	1.50037
RP-8 endcapped	5 μm		1.50033	1.50035

Hibar^® RT Column 4.6 mm i.d.

Modification	Particle size	100-4.6	125-4.6	150-4.6	250-4.6
RP-18 endcapped	3 μm	1.50469		1.50470	1.50471
RP-18 endcapped	5 μm	1.50622	1.51914	1.51455	1.51456
RP-8 endcapped	5 μm	1.51917	1.51916	1.51453	1.51454
Phenyl	5 μm			1.51919	1.51918
NH2	5 μm				1.51913
Si	5 μm				1.51911

Hibar^® RT Column 10 mm i.d.

Modification	Particle size	250-10
RP-18 endcapped	5 μm	1.51915
Si	5 μm	1.51912

Purospher™ STAR in Hibar^® HR UHPLC columns 2.1 mm i.d.

The Hibar^® HR UHPLC columns are designed for use in UHPLC instruments. The pressure stability is set at 1000 bar.

Figure 3. Purospher™ STAR in Hibar^® HR UHPLC columns

Hibar^® HR UHPLC Columns

Modification	Particle size	30-2.1	50-2.1	100-2.1	150-2.1	250-2.1
RP-18 endcapped	2 μm	1.50645	1.50646	1.50648	1.50649
RP-18 endcapped	3 μm	1.50650	1.50651	1.50653	1.50654	1.50655
RP-8 endcapped	2 μm		1.50630	1.50629
RP-8 endcapped	3 μm		1.50674	1.50675
Phenyl	2 μm		1.51013	1.51014
Phenyl	3 μm		1.50672	1.50673