Selection of Purification Equipment

Simple IEX chromatography, such as elution by a step-gradient, can be performed using a syringe or peristaltic pump with prepacked HiTrap columns. A chromatography system is required in order to achieve a high resolution separation using accurately controlled linear gradient elution, to take advantage of the high flow rates of modern media, or when the same column is to be used for many runs.

Standard ÄKTAdesign configurations

Way of working	Pilot	Explorer 100	Purifier 10	FPLC	Prime	Syringe or peristaltic pump + HiTrap column
Simple, step-gradient elution	✓	✓	✓	✓	✓	✓
Reproducible performance for routine separation	✓	✓	✓	✓	✓
Optimization of one step separation to increase purity	✓	✓	✓	✓	✓
System control and data handling for regulatory requirements, e.g. GLP	✓	✓	✓	✓
Automatic method development and optimization	✓	✓	✓	✓
Automatic buffer preparation	✓	✓	✓
Automatic pH scouting	✓	✓	✓
Automatic media or column scouting	✓	✓
Automatic multi-step purification	✓	✓
Scale up, process development and transfer to production	✓	✓
Proven sanitary design for cGMP with easily exchanged wetted parts	✓

Figure 1. Standard ÄKTAdesign configurations