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HomeSolid Phase Microextraction (SPME)GC/MS Analysis of Ethanol in Low Alcohol Beer on SUPELCOWAX® 10 after Headspace SPME using a 100 µm PDMS Fiber

GC/MS Analysis of Ethanol in Low Alcohol Beer on SUPELCOWAX® 10 after Headspace SPME using a 100 µm PDMS Fiber

GC/MS Analysis of Ethanol in Low Alcohol Beer on SUPELCOWAX® 10 after Headspace SPME using a 100 μm PDMS Fiber

Conditions

Sample PreparationSample equilibrated prior to extraction for 7 min at 40 °C, with agitation at 250 rpm
Sample/Matrix4 mL of diluted low alcohol beer sample in 10 mL headspace vial
Desorption Process250 °C, 3 min, split 10:1
ExtractionHeadspace at 40C for 2 min, agitation at 250 rpm
SPME fiberPk/3 SPME Assy, 100 µm, PDMS FS 24 ga Auto (Red) (57301)
ColumnSUPELCOWAX 10, 30 m x 0.25 mm I.D., 0.25 µm (24079)
Oven40 °C (5 min), 8 °C/min to 70 °C, 20 °C/min to 250 °C, held for 5 min
Carrier GasHelium, 1 mL/min constant flow
InjectionSplit, 10:1
Liner0.75 mm I.D. SPME
Inj. Temp.250 °C
DetectorMSD
MSD interface250 °C
Scan Rangem/z 25-300

Description

Analysis NoteTraditional methods for determination of alcohol in beverages include specific gravity and gas chromatography (GC). Specific gravity may not be able to accurately measure levels in beverages with low alcohol content, below 1%. GC is a better approach because it does not have this limitation. However, direct injection of samples containing sugars or other nonvolatile matrix components can foul the GC instrument. This drawback of GC can be overcome by sampling the headspace over the sample using solid phase microextraction (SPME), The method shown here was developed specifically for the determination of ethanol in low alcohol content samples, and was used to analyze a reference material of low alcohol beer. The sample had a certified value of 0.505% alcohol by volume (% ABV). Using headspace SPME, the average value measured was 0.495% ABV, representing 97% accuracy.
Featured IndustryFood and Beverages
Legal InformationSUPELCOWAX is a registered trademark of Sigma-Aldrich Co. LLC
Other NotesSPME Sample pretreatment: Dilute 400 uL of sample to 4 mL with 0.1M sodium phosphate buffer ( pH 7) containing 25% NaCl
SuitabilityApplication for SPME, application for GC/MS
Materials
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