HPLC Analysis of Thalidomide Enantiomers on Astec® CHIROBIOTIC® V
CONDITIONS
column
CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)
mobile phase
[A] 18 mM ammonium nitrate, pH 5.5; [B] acetonitrile; (65:35, A:B)
flow rate
1 mL/min
column temp.
22 °C
detector
UV, 240 nm
injection
20 μL
sample
1 mg/mL in methanol
Beschreibung
Rechtliche Hinweise
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC