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HPLC Analysis of Spice Cannabinoids JWH-018, JWH-073 and Metabolites on Ascentis® Express C18

HPLC Analysis of Spice Cannabinoids JWH-018, JWH-073 and Metabolites on Ascentis® Express C18 application for HPLC

Materialien

Analytische Säule

Produkt-Nr.
Beschreibung
Preisangaben

Ascentis® Express C18, 2,7 μm HPLC-Säule

2.7 μm particle size, L × I.D. 10 cm × 3 mm

Standard

Produkt-Nr.
Beschreibung
Preisangaben

JWH-018 -Lösung

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

JWH-073 -Lösung

100 μg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

JWH-018 5-Pentanoic acid metabolite solution

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

JWH-018 4-Hydroxypentyl metabolite solution

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

JWH-073 4-Butanoic acid metabolite solution

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

JWH-073 N-(3-hydroxybutyl) metabolite solution

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)

mobile phase

[A] 0.1% formic acid in water; [B] 0.1% formic acid in water:acetonitrile (10:90)

gradient

20% B held for 3 min; 20 to 100% B in 0.1 min; 100% B held for 2 min

flow rate

0.5 mL/min

pressure

2150 psi (148 bar)

column temp.

35 °C

detector

UV, 315 nm

injection

2 μL

sample

17 μg/mL each in acetonitrile

Beschreibung

Hinweis zur Analyse

Synthetic cannabinoids (e.g. "Spice") are a type of designer drug that provides a cannabis-type high. New synthetic cannabinoids are continually being introduced as suppliers tweak the molecular structures. The ability to rapidly and reliably identify the continually changing population of these compounds is a significant analytical challenge facing forensic chemists. A rapid separation of six of these compounds on an Ascentis Express C18 column is shown here. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Rechtliche Hinweise

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany