HPLC Analysis of Phenolic Compounds on Ascentis® Express 2.7 μm RP-Amide
Materialien
CONDITIONS
column
Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 2.7 μm particles (53929-U)
mobile phase
[A] 20 mM phosphoric acid, pH 2; [B] acetonitrile; (75:25, A:B)
flow rate
1.5 mL/min
column temp.
35 °C
detector
UV, 270 nm
injection
25 μL
sample
50 mg/L in mobile phase
Beschreibung
Hinweis zur Analyse
Chromatography of Seven Phenol Compounds on Ascentis Express RPA
Rechtliche Hinweise
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany