HPLC Analysis of Beverage Additives in Diet Cola on Ascentis® Express C18
CONDITIONS
column
Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)
mobile phase
[A] 20 mM ammonium acetate, pH 4.7 with acetic acid: [B] acetonitrile; (90:10, A:B)
flow rate
0.6 mL/min
column temp.
40 °C
detector
UV, 214 nm
injection
1 μL
sample
100 - 500 μg/mL in 95:5, buffer: acetonitrile
Beschreibung
Hinweis zur Analyse
This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of common drink additives used in diet soda.
Rechtliche Hinweise
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany