Direkt zum Inhalt
Merck

HPLC Analysis of an Energy Drink on Ascentis® Express HILIC (UV Detection)

HPLC Analysis of an Energy Drink on Ascentis® Express HILIC (UV Detection) application for HPLC

Materialien

Analytische Säule

Produkt-Nr.
Beschreibung
Preisangaben

53970-U

Ascentis® Express HILIC HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 3 mm

Standard

Produkt-Nr.
Beschreibung
Preisangaben

C1778

Koffein

Sigma Reference Standard, vial of 250 mg

S1626

Sorbinsäure

99.0-101.0% anhydrous basis

47862

Pyridoxinhydrochlorid (B6)

analytical standard

47865-U

Nicotinamid (Niacinamid)

analytical standard

47861

Riboflavin (B2)

analytical standard

8S61336

SS Benzoesäure

2000 μg/mL in dichloromethane, analytical standard

CONDITIONS

column

Ascentis Express HILlC, 10 cm x 3.0 mm I.D., 2.7 μm particles (53970-U)

mobile phase

[A] 100 mM ammonium acetate, pH 5.0 with acetic acid; [B] water; [C] acetonitrile; (09:01:90, A:B:C)

flow rate

0.6 mL/min

pressure

812 psi (56 bar)

column temp.

35 °C

detector

UV, 254 nm

injection

2 μL

sample

Dilute 1:9 in acetonitrile

Beschreibung

Hinweis zur Analyse

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of common drink additives used in caffeinated energy drinks. The analysis was performed with UVand ELSD detectors in series, this chromatogram being from the UV.

Rechtliche Hinweise

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS