The NF-YC-RGL2 module integrates GA and ABA signalling to regulate seed germination in Arabidopsis.

The NF-YC-RGL2 module integrates GA and ABA signalling to regulate seed germination in Arabidopsis.

Nature communications (2016-09-15)

Xu Liu, Pengwei Hu, Mingkun Huang, Yang Tang, Yuge Li, Ling Li, Xingliang Hou

ZUSAMMENFASSUNG

The antagonistic crosstalk between gibberellic acid (GA) and abscisic acid (ABA) plays a pivotal role in the modulation of seed germination. However, the molecular mechanism of such phytohormone interaction remains largely elusive. Here we show that three Arabidopsis NUCLEAR FACTOR-Y C (NF-YC) homologues NF-YC3, NF-YC4 and NF-YC9 redundantly modulate GA- and ABA-mediated seed germination. These NF-YCs interact with the DELLA protein RGL2, a key repressor of GA signalling. The NF-YC-RGL2 module targets ABI5, a gene encoding a core component of ABA signalling, via specific CCAAT elements and collectively regulates a set of GA- and ABA-responsive genes, thus controlling germination. These results suggest that the NF-YC-RGL2-ABI5 module integrates GA and ABA signalling pathways during seed germination.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

F3165

Sigma-Aldrich

Monoklonaler ANTI-FLAG^® M2-Antikörper in Maus hergestellte Antikörper, clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

16-201

Sigma-Aldrich

Protein-G-Agarose/Lachssperma-DNA, 2,5 ml, for use in chromatin immunoprecipitations (ChIP assays)

IP10

Millipore

Protein G Plus/Protein A-Agarose, A mixture of Protein G PLUS and Protein A covalently conjugated to agarose. Useful for purification of IgG from biological fluids.