- Piezoelectric-excited millimeter-sized cantilever biosensors.
Piezoelectric-excited millimeter-sized cantilever biosensors.
Methods in molecular biology (Clifton, N.J.) (2009-01-23)
Raj Mutharasan
PMID19159091
ZUSAMMENFASSUNG
In this chapter, method of fabricating a cantilever biosensors and their use in measuring the presence of a protein is described. There are many variations in construction of a cantilever sensor. A simple and an easy version is described in this chapter. The specificity of the sensor is obtained by immobilizing an antibody specific to the antigen of interest. The piezoelectric cantilever sensors are very sensitive and can easily detect a 60 kDa protein at 100 fg/mL concentration. Experimental procedure for carrying out detection of a target analyte is outlined and a sample set of results is included.