G005680

HPLC Analysis of Biogenic Amines on Ascentis^® RP-Amide

application for HPLC

Eigenschaften

• Methode(n): HPLC: suitable
• Testparameter: column: Ascentis RP-Amide, 15 cm x 2.1 mm I.D., 5 μm particles (565305-U); mobile phase: [A] 0.1% ammonium acetate in water (pH unadjusted); [B] acetonitrile; gradient: 25 to 75% B in 10 min; held at 75% B for 5 min; flow rate: 0.5 mL/min; column temp.: 35 °C; detector: UV, 340 nm; injection: 10 μL
• Eignung: application for HPLC
• Anwendung(en): clinical

Beschreibung

Hinweis zur Analyse

Following pre-column derivatization of primary amino groups using o-phthaldialdehyde/thiol reagent, the Ascentis RP-Amide was used for the analysis of biogenic amines commonly found in wines. These include histamine, spermine, spermidine, tyramine, putrescine and cadaverine. Structures along with an optimized chromatogram are presented.

Rechtliche Hinweise

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyt

Histamine
Spermine
Spermidine
Tyramine
1,4-Diaminobutane
Cadaverine