T7777

Tris-Glycin-SDS Puffer als 10× Konzentrat

• Synonym(e): TRIS–glycin–SDS-Puffer -Lösung
• UNSPSC-Code: 41105319
• eCl@ss: 32129211
• NACRES: NA.25

Eigenschaften

• Sterilität: 0.2 μm filtered
• Form: liquid
• Anwendung(en): diagnostic assay manufacturing
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both anode and cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.

Anwendung

Tris-glycine-SDS buffer 10× concentrate has been used as a running buffer in sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE). It is also used to block membranes with 5% non-fat dry milk for western blotting. TGS is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.

Rekonstituierung

Dilution of the 10× TGS buffer produces a 1× running buffer containing 25 mM Tris, 192 mM glycine and 0.1% SDS, pH approx. 8.6.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type ABEK (EN14387) respirator filter