SRP3042

FGF-acidic human

Animal-component free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

• Synonym(e): ECGF-beta, FGF-1, Fibroblast Growth Factor-acidic, HBGF-1
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in E. coli
• Assay: ≥95% (HPLC); ≥95% (SDS-PAGE)
• Form: lyophilized
• Wirksamkeit: ≥2x10^6 units/mg
• Mol-Gew.: 15.8 kDa
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: <0.1 EU/μg endotoxin, tested
• Farbe: white to off-white
• UniProt-Hinterlegungsnummer: P05230
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... FGF1(2246)

Beschreibung

Allgemeine Beschreibung

FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant human FGF-acidic is a 15.8 kDa protein consisting of 140 amino acid residues.

Biochem./physiol. Wirkung

FGF-acidic is one of 23 known members of the FGF family. Recombinant human FGF-acidic is a 15.8 kDa protein consisting of 140 amino acid residues.

Sequenz

MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

Physikalische Form

Lyophilized from 5 mM Sodium Phosphate, pH 8.0 + 100 mM NaCl.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in 5 mM Sodium Phosphate, pH 8.0 to a concentration of <0.5 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable