SAB4200106

Anti-ULK1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(e): Anti-ATG1 autophagy related 1 homolog, Anti-UNC51, Anti-Unc51.1, Anti-unc-51-like kinase 1
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~150 kDa
• Speziesreaktivität: human
• Erweiterte Validierung: recombinant expression; Learn more about Antibody Enhanced Validation
• Konzentration: ~1.0 mg/mL
• Methode(n): western blot: 1-2 μg/mL using whole extracts of HEK-293T cells overexpressing human ULK1.
• UniProt-Hinterlegungsnummer: O75385
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... ULK1(8408)

Beschreibung

Allgemeine Beschreibung

Uncoordinated 51-like kinase 1 (ULK1) is expressed in skeletal muscle, heart, pancreas, brain, placenta, liver, kidney and lung. This gene is located on human chromosome 2q24.3. ULK1 localizes to cytoplasmic structures. ULK1 and ULK2 is a homolog of autophagy related 1 (Atg1) gene.

Spezifität

Anti-ULK1 recognizes human ULK1.

Anwendung

Anti-ULK1 has been used in immunoblotting.

Biochem./physiol. Wirkung

Uncoordinated 51-like kinase 1 (ULK1) is involved in modulating Atg9 subcellular dynamics. Knockdown of ULK1, but not ULK2, inhibits the autophagic response. Overexpression of ULK1 also inhibits autophagy, suggesting that ULK1 may have multiple mechanisms for regulating autophagy. Knockdown of Ulk1 shRNA can prevent gastric cancer cell survival.

Physikalische Form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable