P8997

Anti-Purinergic Receptor P2X₇ (extracellular)−FITC antibody produced in rabbit

affinity isolated antibody, lyophilized powder

• Synonym(e): Anti-P2Z
• MDL-Nummer: MFCD06798528
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Konjugat: FITC conjugate
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: lyophilized powder
• Speziesreaktivität: human, rat, mouse
• Methode(n): flow cytometry: 10 μg/test using Jurkat T-cells
• UniProt-Hinterlegungsnummer: Q99572
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... P2RX7(5027) ; mouse ... P2rx7(18439) ; rat ... P2rx7(29665)

Beschreibung

Allgemeine Beschreibung

Purinergic Receptor P2X7 is a ionotrophic ATP-gated channel responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. ATP activation of the P2RX7 leads to recruitment of pannexin pores which allow small molecules such as ATP to leak out of cells further activating of purinergic receptors and physiological responses such a spreading cytoplasmic waves of calcium.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.

Spezifität

Rabbit polyclonal anti-Purinergic Receptor P2X7 (extracellular)−FITC antibody reacts with rat, human, and mouse purinergic receptor P2X7 (extracellular).

Immunogen

synthetic peptide corresponding to residues 136-152 of mouse P2X₇ receptor. Sequence homology with human, mouse, and rat is identical. Bovine is 14/17 residues identical.

Anwendung

Rabbit polyclonal anti-Purinergic Receptor P2X7 (extracellular)−FITC antibody is used to tag Purinergic Receptor P2X7 (extracellular) for detection and quantitation by fluorescence-based immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of Purinergic Receptor P2X7 (extracellular) in ATP-dependent processes such as cell lysis and calcium movement.

Physikalische Form

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 13 - Non Combustible Solids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable