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Merck

M5899

Sigma-Aldrich

Anti-Maus-IgG (Gesamtmolekül)

whole antiserum

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

goat

Konjugat

unconjugated

Antikörperform

whole antiserum

Antikörper-Produkttyp

secondary antibodies

Klon

polyclonal

Enthält

15 mM sodium azide

Methode(n)

indirect ELISA: 1:70,000
quantitative precipitin assay: 3.2-4.8 mg/mL

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

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Allgemeine Beschreibung

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

Spezifität

The antiserum is determined to be immunospecific for mouse IgG by immunoelectrophoresis (IEP) against normal mouse serum and purified mouse IgG.

Anwendung

Anti-Mouse IgG (whole molecule) antibody produced in goat has been used as secondary antibody in:
  • immunoblotting
  • immunohistochemistry
  • proteomic microarrays
Anti-Mouse IgG (whole molecule) antibody produced in goat has been used in:
  • the preparation of immunochromatography strips for magneto-enzyme lateral flow immunoassay
  • immunochromatography
  • MYCN DNA-binding analysis (ChIP-seq)

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)

Biochem./physiol. Wirkung

IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Immunoglobulin G (IgG) helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Qualität

The antiserum has been treated to remove lipoproteins.

Physikalische Form

Supplied as a liquid containing 15 mM sodium azide as preservative.

Lagerung und Haltbarkeit

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

R Holtappels et al.
Journal of virology, 74(4), 1871-1884 (2000-01-22)
Several early genes of murine cytomegalovirus (MCMV) encode proteins that mediate immune evasion by interference with the major histocompatibility complex class I (MHC-I) pathway of antigen presentation to cytolytic T lymphocytes (CTL). Specifically, the m152 gene product gp37/40 causes retention
David J Duffy et al.
Oncotarget, 7(37), 60310-60331 (2016-08-18)
Wnt signalling is involved in the formation, metastasis and relapse of a wide array of cancers. However, there is ongoing debate as to whether activation or inhibition of the pathway holds the most promise as a therapeutic treatment for cancer
The Laboratory Rat (2005)
Tien V Tran et al.
PeerJ, 7, e7779-e7779 (2019-10-04)
Dengue infection represents a global health issue of growing importance. Dengue non-structural protein 1 (NS1) plays a central role in the early detection of the disease. The most common method for NS1 detection is testing by lateral flow immunoassays (LFIAs)
Natascha Krömmelbein et al.
Viruses, 8(2) (2016-02-06)
The human cytomegalovirus (HCMV) replicates to high titers in primary human fibroblast cell cultures. A variety of primary human cells and some tumor-derived cell lines do also support permissive HCMV replication, yet at low levels. Cell lines established by transfection

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