I2276

Interleukin-12 human

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

• Synonym(e): IL-12
• MDL-Nummer: MFCD00212975
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 200
• Rekombinant: expressed in baculovirus infected Sf21 cells
• Assay: ≥97% (SDS-PAGE and N-terminal analysis)
• Form: lyophilized powder
• Wirksamkeit: 0.01-0.05 ng/mL ED₅₀
• Mol-Gew.: glycoprotein 75 kDa
• Verpackung: pkg of 5 μg
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: endotoxin, tested
• UniProt-Hinterlegungsnummer: P29459,; P29460
• Lagertemp.: −20°C
• Angaben zum Gen: human ... IL12A(3592) , IL12B(3593)

Beschreibung

Biochem./physiol. Wirkung

Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)