NCI-H929

95050415

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human unknown/unspecified
• Wachstumsmodus: Suspension
• Karyotyp: Near-tetraploid (8q+)
• Morphologie: Lymphoblastoid
• Produkte: Immunoglobulin A (IgA)
• Rezeptoren: Transferrin, CD38
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian IgA-producing plasmacytoma

Beschreibung der Zelllinie

This IgA-producing cell line has been established from a malignant effusion in a 62-year-old Caucasian woman with myeloma. They are positive for plasma cell antigen 1 (PCA-1), transferrin receptor and CD38 but negative for early B cell developmental markers. HLA DR and CALLA. NCI-H929 is negative for Epstein-Barr virus nuclear antigen. A rearrangement of the c-myc proto-oncogene has been observed as well as the expression of c-myc RNA and an activated ras allele.

Anwendung

Chemotherapeutic agent studies, receptor studies, carcinogenesis assays.

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 9,13
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8,11
vWA: 14,15

Nährmedium

RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 2-4 x 100,000 cells/ml; 5% CO₂; 37°C.

Sonstige Hinweise

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