KG1a

91030101, human bone marrow, Lymphoblast

• UNSPSC-Code: 41106514

Eigenschaften

• product name: KG1a, 91030101
• Biologische Quelle: human bone marrow
• Wachstumsmodus: Suspension
• Karyotyp: 2n = 46, pseudodiploid
• Morphologie: Lymphoblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian bone marrow acute myelogenous leukaemia

Beschreibung der Zelllinie

Derived from KG-1, however KG1a is unresponsive to colony stimulating factor in soft agar culture and does not express the Ia-like antigen. KG1a is resistant to phorbol-diester induced macrophage differentiation and cell proliferation. KG1a cells are morphologically, cytochemically and functionally less mature than the parental KG-1. Expresses HLA A30,31; B35; CW4.

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19

Nährmedium

IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 5x100,000 to 1,000,000 cells/ml. Do not culture below 100,000 cells/ml; 5% CO₂; 37°C.

Sonstige Hinweise

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