G-402

90112715, human kidney, Epithelial-like

• UNSPSC-Code: 41106514

Eigenschaften

• product name: G-402, 90112715
• Biologische Quelle: human kidney
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 47
• Morphologie: Epithelial-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian renal leiomyoblastoma

Beschreibung der Zelllinie

Derived from a tumour of a 9 month old female Caucasian. Highly transformed and grows in soft agar.

Anwendung

Transformation and tumourigenicity studies

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 9,12
D16S539: 11,13
D5S818: 11,12
D7S820: 9,10
THO1: 7,9
TPOX: 10
vWA: 13,17

Nährmedium

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Change medium every 4 days.

Sonstige Hinweise

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