IgH-2

90030804, lizard cardiovascular , Epithelial-like

• UNSPSC-Code: 41106514

Eigenschaften

• product name: IgH-2, 90030804
• Biologische Quelle: lizard cardiovascular
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 34
• Morphologie: Epithelial-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Iguana heart

Beschreibung der Zelllinie

From the heart of a normal immature male Iguana. Cells can support the replication of herpes simplex, PRV, vaccinia and Iguana viruses at 36°C. The only reported reptilian line to be established and serially propagated at an incubation temperature as high as 36°C.

Anwendung

Virus replication studies: HSV, PRV, vaccinia and Iguana viruses

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 36°C.

Sonstige Hinweise

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