37294
Dihydrofolatreduktase aus Rinderleber
~8 U/mL, ammonium sulfate suspension, off-white
Synonym(e):
Tetrahydrofolat-Dehydrogenase
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Alle Fotos(1)
About This Item
CAS-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.54
Empfohlene Produkte
Biologische Quelle
bovine liver
Qualitätsniveau
Form
ammonium sulfate suspension
Spezifische Aktivität
≥6 U/mg protein
Konzentration
~8 U/mL
Farbe
off-white
Dichte
1.2 g/mL at 20 °C
Lagertemp.
2-8°C
Anwendung
Dihydrofolate reductase (DHFR) is involved in the synthesis of purines, thymidine and glycine in folate metabolism. DHFR, from bovine liver, is used for methotrexate analysis . DHFR is useful to study various cancers such as sarcomas, leukemias and head and neck cancers .
Biochem./physiol. Wirkung
Dihydrofolate reductase (DHFR) reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as an electron donor. The binding of antitumor agents, such as methotrexate, to DHFR prevents the formation of reduced folates, which are essential for DNA synthesis .
Einheitendefinition
1 U entspricht der Enzymmenge, die 1 μmol 7,8-Dihydrofolat und NADPH zu 5,6,7,8-Tetrahydrofolat und NADP pro Minute bei pH 6.5 und 25°C umsetzt
Physikalische Form
Suspension in 3.6 M ammonium sulfate solution, pH 7.0
Sonstige Hinweise
In folate metabolism for the synthesis of purines, thymidine and glycine
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves
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Use of dihydrofolate reductase from bovine liver, rather than from L. casei, for determining methotrexate.
R J Brooks
Clinical chemistry, 24(3), 518-519 (1978-03-01)
M A Pesce et al.
Clinical chemistry, 27(3), 380-384 (1981-03-01)
Methotrexate was determined by the homogeneous enzyme immunoassay (EMIT) with the Multistat and CentrifiChem centrifugal analyzers and by the enzyme inhibition assay with use of the Multistat centrifugal analyzer. With both methods, the standard curve extends from 0.2 to 2.0
H B Eastman et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(19), 8572-8576 (1991-10-01)
Dihydrofolate reductase (DHFR; EC 1.5.1.3) is required in folate metabolism for the synthesis of purines, thymidine, and glycine. Although there have been several reports of induction of DHFR enzyme by methotrexate (MTX), a drug that competitively inhibits DHFR, there are
Luca Freschi et al.
Molecular bioSystems, 9(1), 36-43 (2012-10-27)
One of the most important challenges in systems biology is to understand how cells respond to genetic and environmental perturbations. Here we show that the yeast DHFR-PCA, coupled with high-resolution growth profiling (DHFR-qPCA), is a straightforward assay to study the
Inbar Avraham et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(52), E3678-E3686 (2012-12-01)
Plasmodium falciparum causes the deadliest form of human malaria. Its virulence is attributed to its ability to modify the infected RBC and to evade human immune attack through antigenic variation. Antigenic variation is achieved through tight regulation of antigenic switches
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