MABE1529

Anti-BRD7 Antibody, clone BRM 2D3

clone BRM203, from rat

• Synonym(e): BRD7, Bromodomain-containing protein 7, 75 kDa bromodomain protein, Protein CELTIX-1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702

Eigenschaften

• Biologische Quelle: rat
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: BRM203, monoclonal
• Speziesreaktivität: human
• Methode(n): immunoprecipitation (IP): suitable; western blot: suitable
• Isotyp: IgG2aκ
• NCBI-Hinterlegungsnummer: NP_001167455
• UniProt-Hinterlegungsnummer: Q9NPI1
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... BRD7(29117)

Beschreibung

Allgemeine Beschreibung

Bromodomain-containing protein 7 (UniProt Q9NPI1; also known as 75 kDa bromodomain protein, Protein CELTIX-1) is encoded by the BRD7 (also known as BP75, CELTIX1) gene (Gene ID 29117) in human. BRD7 is a ubiquitously expressed single bromodomain-/BRD-containing member of the family of BRD-containing proteins. BRD7 functions as a subunit of the SWI/SNF chromatin-remodeling complex involved in transcription regulation. It also interacts with the well known tumor suppressor p53 to trans-activate genes involved in cell cycle arrest. BRD7 is downregulated in colorectal carcinoma and nasopharyngeal carcinoma, and BRD7 overexpression is reported to inhibit nasopharyngeal carcinoma cell proliferation via cell cycle arrest by transcriptionally regulating ras/MEK/ERK, Rb/E2F, b-catenin and ERK pathways. BRD7 mRNA has been identified as a silencing target of miR-300, overexpression of miR-300 promotes the proliferation, invasion and EMT of osteosarcoma cells through targeting BRD7 mRNA.

Spezifität

Clone BRM 2D3 detects human 75 kDa bromodomain protein, also known as protein CELTIX-1.

Immunogen

Recombinant human BRD7.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Chromatin-Biologie (ChIP)

This Anti-BRD7 Antibody, clone BRM 2D3 is validated for use in Western Blotting, Immunoprecipitation for the detection of BRD7.

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected BRD7 in 10 µg of HEK293 and HeLa cell lysates.
Immunoprecipitation Analysis: A representative lot immunoprecipitated BRD7 from H1299 cell lysate (Courtesy of Thomas Dobner, Institut fur Medizinische Mikrobiologie und Hygiene, Universitat Regensburg, D-93053 Regensburg, Germany).

Qualität

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected BRD7 in 10 µg of Jurkat cell lysate.

Zielbeschreibung

~75 kDa observed. 74.14 kDa (isoform 1), 74.27 kDa (isoform 2) calculated. Added Uncharacterized band(s) may appear in some lysates.

Verlinkung

Replaces: MABE66

Physikalische Form

Protein G purified.

Format: Purified

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable