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Key Documents

525320

Sigma-Aldrich

PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate

liquid, clone PY20, Calbiochem®

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.43

Biologische Quelle

mouse

Qualitätsniveau

100

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

PY20, monoclonal

Form

liquid

Enthält

0.1% Proclin as preservative

Speziesreaktivität (Voraussage durch Homologie)

all

Hersteller/Markenname

Calbiochem®

Lagerbedingungen

OK to freeze

Isotyp

IgG2b

Versandbedingung

wet ice

Lagertemp.

2-8°C

Posttranslationale Modifikation Target

phosphorylation (pTyr)

Angaben zum Gen

human ... PID1(55022)

Allgemeine Beschreibung

Peroxidase conjugate of Anti-Phosphotyrosine Mouse mAb (PY20), Cat. No. 525295. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
Purified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes a broad spectrum of tyrosine-phosphorylated proteins.
This PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate is validated for use in ELISA, Immunoblotting, Immunohistochemistry for the detection of Phosphotyrosine.

Immunogen

KLH conjugated to phosphotyrosine, glycine, and alanine

Anwendung

ELISA (1:2000)

Immunoblotting (1:500-1:2000)

Immunohistochemistry (1:500)

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

In PBS, 50% glycerol, 1% BSA, pH 7.4.

Sonstige Hinweise

Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.
Specific for tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine; does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosine containing proteins and will adsorb the antibody. Use a buffer with <150 mM NaCl and divalent cations not much >1 mM because this antibody′s binding is inhibited by high salt concentrations and divalent cations. Variables associated with assay conditions will dictate the proper working dilution.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Andrew J Garton et al.
Cancer research, 66(2), 1015-1024 (2006-01-21)
OSI-930 is a novel inhibitor of the receptor tyrosine kinases Kit and kinase insert domain receptor (KDR), which is currently being evaluated in clinical studies. OSI-930 selectively inhibits Kit and KDR with similar potency in intact cells and also inhibits
Justin M Scheer et al.
PloS one, 7(12), e51817-e51817 (2013-01-04)
The structure of the Fab region of antibodies is critical to their function. By introducing single cysteine substitutions into various positions of the heavy and light chains of the Fab region of trastuzumab, a potent antagonist of HER2, and using

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