05-1403

Anti-Giα2 Antibody, clone L5

clone L5, from mouse

• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: L5, monoclonal
• Speziesreaktivität: guinea pig (43%), rat (69%), bovine (100%), human (62%), mouse (69%)
• Methode(n): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotyp: IgG2b
• NCBI-Hinterlegungsnummer: NM_002070.2
• UniProt-Hinterlegungsnummer: P04899
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... GNAI2(2771)

Beschreibung

Spezifität

Specificity confirmed by Western blot versus bovine Gαt, rat Goα, rat Giα-1, Giα-2, rat Gia-3, bovine Gsα, and murine GoαA and GoαB.

This antibody recognizes Giα-2.

Immunogen

Recombinant Giα-2, purified by the method of Linder (J. Biol. Chem. [1990] 265, 8243).

Anwendung

Research Category
Zelluläre Signaltransduktion

Research Sub Category
GPCR-, cAMP/cGMP- & kalziumabhängige Signalübertragung

Optimal working dilutions must be determined by end user.

Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of G-Protein Monoclonal Antibody: Testicular Cancer

Western Blot: A previous lot of this antibody was used at 1:500 - 1:1000, with detection by 125l-labeled anti-IgG. Labels a single protein band of 39 - 42 kDa in bovine and rat membranes

This Anti-Giα2 Antibody, clone L5 is validated for use in WB, IH(P) for the detection of Giα2.

Qualität

Routinely evaluated by Western Blot on Huvec lysates.

Western Blot Analysis: 1:500 dilution of this lot detected G I ALPHA 2 on 10 μg of Huvec lysates.

Zielbeschreibung

Labels a single protein band of 39-42 kDa in bovine and rat membranes.

Verlinkung

Replaces: MAB3077

Physikalische Form

Purified

Format: Purified

Purified mouse monoclonal IgG2b, in 0.02 M phosphate buffer, 0.25 M sodium chloride, pH 7.6, with 0.1% sodium azide.

Lagerung und Haltbarkeit

Maintain at 2-8°C for 1 year from date of receipt.

Hinweis zur Analyse

Control
Huvec cell lysate

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable