Analytical Method: Analytical Quality Assurance, Standard for Hydrogen peroxide

Reagents:
Prod. No. 109122 Potassium permanganate solution 0.02 mol/l
Prod. No. 107209 Perhydrol 30 % for analysis
Prod. No. 100716 Sulfuric acid 25 % for analysis
Prod. No. 116754 Water for analysis

Preparation of a stock solution:
Place 10 mL of Perhydrol 30 % in a calibrated or conformity-checked 100-mL volumetric flask and make up to the mark with distilled water. Transfer 30 mL (full pipette) of this solution to a calibrated or conformity-checked
1000-mL volumetric flask and make up to the mark with distilled water. The stock solution prepared according to this procedure has a concentration of approximately 1000 mg/L hydrogen peroxide.

Precise assay of the standard solution (stock solution) for hydrogen peroxide:
Pipette 50.0 mL (full pipette) of the hydrogen peroxide stock solution of approx. 1000 mg/Linto a 500-mL conical flask, dilute with 200 mL of distilled water, and add 30 mL of sulfuric acid 25 %. Titrate with a 0.02 mol/L potassium permanganate solution until the color changes to pink.

Calculation of the exact content of the hydrogen peroxide concentration:
Consumption of potassium permanganate (mL) x 34.02 = content of hydrogen peroxide, in mg/L

Further investigational concentrations may be prepared from the stock solution exactly determined according to the procedure described above by diluting accordingly.

Stability:
When stored in a cool place (refrigerator), the stock solution and the further diluted investigational solutions remain stable for one day.

For use with test kits:		Measuring range in mg/L
110011	MQuantTM Peroxide Test	5 - 50 H2O2
110081	MQuantTM Peroxide Test	1 - 100 H2O2
110337	MQuantTM Peroxide Test	100 - 1000 H2O2
116974	Reflectoquant® Peroxide Test	0.2 - 20.0 H2O2
116731	Reflectoquant® Peroxide Test	100 - 1000 H2O2
114731	Spectroquant® Hydrogen Peroxide Cell Test	2.0 - 20.0 H2O2 0.25 - 5.00 H2O2
118789	Spectroquant® Hydrogen Peroxide Test	0.15 - 6.00 H2O2