HPLC Analysis of Triazole Antifungal Agent Enantiomers on Astec® CYCLOBOND I 2000 HP-RSP
CONDITIONS
column
Astec CYCLOBOND I 2000 HP-RSP, 25 cm x 4.6 mm I.D., 5 μm particles (24024AST)
mobile phase
[A] 0.1% formic acid; [B] acetonitrile; (70:30, A:B)
flow rate
1 mL/min
detector
UV, 230 nm
injection
1 μL
sample
0.1 mg/mL in methanol
peaks 1&2: enilconazole
peaks 2&3: eberconazole
peaks 4&5: miconazole
peaks 6&7: ketoconazole
Description
Analysis Note
Note: Peak 2 of enilconzaole coelutes with peak 1 of eberconazole under these conditions.
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
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