HPLC Analysis of Hydrobenzoin Enantiomers on Astec® CYCLOBOND I 2000
CONDITIONS
column
CYCLOBOND I 2000, 25 cm x 4.6 mm I.D., 5 μm particles (20024AST)
mobile phase
[A] 20 mM ammonium acetate, pH 4.1; [B] acetonitrile; (80:20, A:B)
flow rate
0.9 mL/min
column temp.
25 °C
detector
UV, 254 nm
injection
10 μL
sample
5 mg/mL in methanol
Description
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
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