HPLC Analysis of FMOC-Proline Enantiomers on Astec^® CYCLOBOND^™ I 2000 SN

column

CYCLOBOND I 2000 SN, 25 cm x 4.6 mm I.D., 5 μm particles (20524AST)

mobile phase

[A] acetonitrile; [B] methanol; [C] acetic acid; [D] ammonium hydroxide; (85:15:0.4:0.1, A:B:C:D)

flow rate

0.9 mL/min

column temp.

25 °C

detector

UV, 254 nm

injection

5 μL

sample

2 mg/mL in methanol